College of Sciences, Nanjing University of Technology, Nanjing 210009, PR China.
Anal Chim Acta. 2012 Apr 20;723:94-100. doi: 10.1016/j.aca.2012.02.032. Epub 2012 Feb 24.
In this paper, a three dimensional ordered macroporous self-doped polyaniline/Prussian blue (3DOM SPAN/PB) bicomponent film was fabricated via the inverted crystal template technique using step-by-step electrodeposition. In this bicomponent film, PB not only acted as a redox mediator, but also presented increased stability in neutral or weak alkaline solution by the protection of SPAN layer on the top. A novel glucose biosensor was fabricated based on the large active surface area and excellent conductivity possessed by the 3DOM SPAN/PB film. The applying experimental conditions of the glucose biosensor have been optimized. Under the optimal conditions, the biosensor showed a wide linear range over three orders of magnitude in glucose concentrations (from 2 to 1600 μM) and a low detection limit of 0.4 μM. Moreover, the biosensor exhibited short response time, high selectivity and excellent operation stability, which can be applied to detect the blood sugar in real samples without any pretreatment.
本文采用分步电沉积法通过反相晶体模板技术制备了三维有序大孔自掺杂聚苯胺/普鲁士蓝（3DOM SPAN/PB）双组分膜。在这种双组分膜中，PB 不仅充当氧化还原介体，而且通过顶部 SPAN 层的保护，在中性或弱碱性溶液中呈现出更高的稳定性。基于 3DOM SPAN/PB 薄膜具有较大的活性表面积和优异的导电性，制备了一种新型葡萄糖生物传感器。优化了葡萄糖生物传感器的应用实验条件。在最佳条件下，该生物传感器在葡萄糖浓度（2 至 1600 μM）的三个数量级范围内表现出宽线性范围和低检测限 0.4 μM。此外，该生物传感器具有较短的响应时间、高选择性和出色的操作稳定性，可用于检测实际样品中的血糖，无需任何预处理。
