College of Veterinary Science, Khanapara, Guwahati, India.
Pharm Biol. 2012 Jul;50(7):892-9. doi: 10.3109/13880209.2011.642885. Epub 2012 Apr 6.
The floral richness of the North-East Indian region cannot be neglected in context to its medicinal importance. Achyranthes aspera Linn. (Amaranthaceae; Prickly Chaff flower) is an indigenous plant species of this region. Although the local traditional healers have ethnomedical knowledge on the use of this plant, there is no scientific study on wound-healing activity of this plant.
The healing efficacy of methanol leaf extract of A. aspera (MEAA) in granulation tissue of burn wound and its antioxidant activity are investigated.
Methanol extract of leaves of A. aspera was used for compounding 5% (w/w) ointment, which was applied topically twice daily in experimental burn wound in rats. Healing potential was assessed by rate of wound contraction, antioxidant and biochemical assay which was supported by gelatin zymography and histopathology.
In the present study, 5% ointment of A. aspera showed significant (p < 0.05) wound healing, which was evident by wound contraction, elevation of various antioxidant enzymes viz. SOD, catalase, vitamin C and prohealing and biochemical parameters like hydroxyproline and protein content than the control animals. Up-regulated expression of matrix metalloproteinases (MMP-2 and 9) was also observed by gelatin zymography. Histopathological examination of the granulation tissues in the A. aspera-treated animals showed collagen deposition, fibroblast proliferation and formation of epidermis.
The methanol leaf extract of A. aspera showed excellent wound-healing activities which has great potential for development of plant-based product.
在考虑到其药用重要性的情况下，不能忽视东北印度地区丰富的花卉资源。反枝苋（苋科；刺苋菜）是该地区的一种本土植物物种。尽管当地的传统治疗师对该植物的民间医学用途有一定的了解，但目前还没有关于该植物在伤口愈合方面的科学研究。
研究反枝苋甲醇叶提取物（MEAA）对烧伤创面肉芽组织的愈合作用及其抗氧化活性。
采用反枝苋叶甲醇提取物制备 5%（w/w）软膏，每日两次局部涂抹于大鼠实验性烧伤创面。通过创面收缩率、抗氧化和生化测定来评估愈合潜力，其中凝胶电泳和组织病理学支持抗氧化和生化测定。
在本研究中，5%的反枝苋软膏表现出显著的（p<0.05）伤口愈合作用，这表现在创面收缩、各种抗氧化酶（如 SOD、过氧化氢酶、维生素 C 和促愈合）以及羟脯氨酸和蛋白质等生化参数的升高上，优于对照组动物。明胶电泳还观察到基质金属蛋白酶（MMP-2 和 9）的表达上调。反枝苋处理动物的肉芽组织组织学检查显示胶原沉积、成纤维细胞增殖和表皮形成。
反枝苋甲醇叶提取物表现出优异的伤口愈合活性，这为开发基于植物的产品提供了巨大的潜力。
