MTCC-Microbial Type Culture Collection & Gene Bank, Institute of Microbial Technology, Council of Scientific and Industrial Research, Chandigarh 160036, India.
Syst Appl Microbiol. 2012 Jul;35(5):320-5. doi: 10.1016/j.syapm.2012.04.004. Epub 2012 May 28.
Novel orange pigmented, Gram-negative-staining, rod-shaped, non-motile, strictly aerobic strains designated NIO-S1(T) and NIO-S2 were isolated from the water sample of a pond adjacent to the coast and an algal mat from a fish pond, respectively, at Kakinada, India. Both strains were positive for oxidase, catalase and β-galactosidase activities. The predominant fatty acids in NIO-S1(T) were iso-C(15:0) (39.6%), anteiso-C(15:0) (9.9%), iso-C(17:0) 3OH (10.9%) and C(16:1)ω7c/C(16:1)ω6c (summed feature 3) (5.7%). The strains contained MK-7 as the major respiratory quinine, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unidentified lipids as the polar lipids. Phylogenetic analysis indicated that strain NIO-S1(T) was a member of the family "Cyclobacteriaceae" of the class "Sphingobacteriia" and it clustered with the genera Fontibacter, Cecembia and Aquiflexum with phylogenetic distances of 6.8, 9.0 and 12.2% (93.2, 91.0 and 87.8% similarity), respectively. DNA-DNA hybridization between strains NIO-S1(T) and NIO-S2 showed a relatedness of 93% and rep-PCR banding patterns were similar. Based on data from the current polyphasic study, it is proposed that the new isolates be placed in a new genus and species with the name Shivajiella indica gen. nov., sp. nov. The type strain of Shivajiella indica is NIO-S1(T) (= KCTC 19812(T)=MTCC 11065(T)).
新型橙色着色、革兰氏阴性染色、杆状、非运动、严格需氧菌株 NIO-S1(T) 和 NIO-S2 分别从印度卡基纳达沿海池塘水样和鱼塘藻垫中分离得到。两株菌均氧化酶、过氧化氢酶和β-半乳糖苷酶活性阳性。NIO-S1(T) 中的主要脂肪酸为异-C(15:0) (39.6%)、anteiso-C(15:0) (9.9%)、iso-C(17:0) 3OH (10.9%) 和 C(16:1)ω7c/C(16:1)ω6c (summed feature 3) (5.7%)。两株菌均含有 MK-7 作为主要呼吸醌，以及双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和三种未鉴定的脂质作为极性脂质。系统发育分析表明，菌株 NIO-S1(T) 是 Sphingobacteriia 纲“Cyclobacteriaceae”科的成员，与 Fontibacter、Cecembia 和 Aquiflexum 属聚类，进化距离分别为 6.8%、9.0%和 12.2%（93.2%、91.0%和 87.8%相似度）。NIO-S1(T) 株与 NIO-S2 株之间的 DNA-DNA 杂交显示相关性为 93%，重复聚合酶链反应（rep-PCR）带型相似。基于当前多相研究的数据，建议将新分离株置于一个新属和新种中，命名为 Shivajiella indica 属。新种 Shivajiella indica 的模式株为 NIO-S1(T)=KCTC 19812(T)=MTCC 11065(T)。
