阿魏酸和白藜芦醇对小鼠链脲佐菌素诱导糖尿病的保护作用。

Protective effect of ferulic acid and resveratrol against alloxan-induced diabetes in mice.

机构信息

Department of Animal Health and Management, Science block, Alagappa University, Karaikudi 630 003, India.

出版信息

Eur J Pharmacol. 2012 Sep 5;690(1-3):226-35. doi: 10.1016/j.ejphar.2012.05.019. Epub 2012 May 31.

DOI:10.1016/j.ejphar.2012.05.019

Abstract

The present study was to investigate the effect of ferulic acid and resveratrol on alloxan-induced diabetic mice, through analysis of basic biochemical parameters, enzymic as well as non-enzymic activities, lipid peroxidation and immunohistochemical studies. Alloxan was administered as a single dose (75 mg/kg body weight) to induce diabetes in mice. A dose of ferulic acid (10 mg/kg body weight) and resveratrol (20 mg/kg body weight) were administrated orally, to the alloxan-induced diabetic mice. The levels of basic biochemical markers and lipid peroxidation were significantly (P<0.05) increased in alloxan-induced diabetic mice. The levels of antioxidants were significantly (P<0.05) decreased in liver, kidney and serum. Immunohistochemical studies in alloxan induced mice demonstrated a marked increase in the immunoreactivity of nuclear transcription factor (NF-κB). Treating the diabetic mice with doses of ferulic acid and resveratrol restored the changes in the above parameters analyzed. The present study, showed that ferulic acid and resveratrol exerted antioxidant as well as anti-diabetic effects, consequently alleviate liver, kidney and pancreas damage caused by alloxan-induced diabetes, probably through inhibition of the proinflammatory factor, NF-κB.

摘要

本研究旨在通过分析基本生化参数、酶和非酶活性、脂质过氧化和免疫组织化学研究，探讨阿魏酸和白藜芦醇对链脲佐菌素诱导的糖尿病小鼠的作用。单次给予链脲佐菌素（75mg/kg 体重）诱导小鼠糖尿病。阿魏酸（10mg/kg 体重）和白藜芦醇（20mg/kg 体重）以口服方式给予链脲佐菌素诱导的糖尿病小鼠。在链脲佐菌素诱导的糖尿病小鼠中，基本生化标志物和脂质过氧化水平显著升高（P<0.05）。肝、肾和血清中抗氧化剂水平显著降低（P<0.05）。免疫组织化学研究在链脲佐菌素诱导的小鼠中表明核转录因子（NF-κB）的免疫反应性明显增加。用阿魏酸和白藜芦醇的剂量治疗糖尿病小鼠，恢复了分析的上述参数的变化。本研究表明，阿魏酸和白藜芦醇具有抗氧化和抗糖尿病作用，从而减轻了阿魏酸诱导的糖尿病引起的肝、肾和胰腺损伤，可能是通过抑制促炎因子 NF-κB 实现的。