Aggarwal Anjali, Chandra Gulab, Singh Anil Kumar
Dairy Cattle Physiology Division, National Dairy Research Institute (NDRI), Karnal, 132001, Haryana, India.
Trop Anim Health Prod. 2013 Jan;45(1):239-45. doi: 10.1007/s11250-012-0196-z. Epub 2012 Jun 15.
The study was conducted to investigating the effect of α-tocopherol acetate on heat shock protein 70 (Hsp70), oxidative stress, and antioxidant status during periparturient period in medium body condition score crossbred cows. Twenty crossbred Karan Fries cows with confirmed pregnancy were selected 2 months before expected date of calving. The cows were randomly distributed in to two groups: 10 cows were kept as control and 10 were supplemented with α-tocopherol acetate during dry period for 2 months. Blood samples were collected at -20, -10, -5, 0, 5, 10, and 20 days in relation to the expected date of calving. Superoxide dismutase, catalase, and total immunoglobulin were significantly higher (P < 0.01) in treatment as compared to control cows. Heat shock protein 70 and thiobarbituric acid reactive substance levels were significantly lower (P < 0.01) in the treatment cows than their counterpart. Treatment with α-tocopherol acetate during dry period resulted in reduced oxidative stress, heat shock protein Hsp70 levels, improved antioxidant, and improved immunity status indicating beneficial effect of α-tocopherol acetate treatment.
本研究旨在调查醋酸α-生育酚对中等体况评分的杂交奶牛围产期热休克蛋白70（Hsp70）、氧化应激和抗氧化状态的影响。在预计产犊日期前2个月，选择20头已确认怀孕的杂交卡兰弗里斯奶牛。将奶牛随机分为两组：10头奶牛作为对照组，10头奶牛在干奶期补充醋酸α-生育酚2个月。在预计产犊日期的前20、10、5、0、5、10和20天采集血样。与对照奶牛相比，处理组奶牛的超氧化物歧化酶、过氧化氢酶和总免疫球蛋白显著更高（P<0.01）。处理组奶牛的热休克蛋白70和硫代巴比妥酸反应物质水平显著低于对照组（P<0.01）。干奶期用醋酸α-生育酚处理可降低氧化应激、热休克蛋白Hsp70水平，改善抗氧化能力和免疫状态，表明醋酸α-生育酚处理具有有益作用。
