Feng Mei, Wang Xing-Hua, Yang Xiao-Bo, Xiao Qing, Jiang Fa-Gang
Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
Int J Ophthalmol. 2012;5(2):151-7. doi: 10.3980/j.issn.2222-3959.2012.02.07. Epub 2012 Apr 18.
To explore the effect of saturated hydrogen saline on blue light-induced retinal damage in rats.
The retinal damage of rats was induced by blue light exposure for 6 hours and examined 8 hours, 16 hours and 24 hours after the exposure. One hundred female Sprague-Dawley rats were randomly divided into four groups. Group 1 included 30 rats received light exposure without any other treatment. Group 2 included 30 rats received light exposure with intraperitoneal injection of normal saline. Group 3 included 30 rats received light exposure with intraperitoneal injection of saturated hydrogen saline. And Group 4 included the other 10 rats which did not receive any treatment. The amount of intraperitoneal injection of saturated hydrogen saline and normal saline was calculated in the ratio of 1ml/100g of rat weight. Specimens were collected and processed by H-E staining, ultrastructure observation, biochemical measurement. Morphological changes were observed by light microscope and transmission electron microscope (TEM) and the retinal outer nuclear layer (ONL) thickness was measured by IPP 6.0, while the malondialdehyde (MDA) was measured by colorimetric determination at 532 nm.
Although the structure of retina in Group 1 and Group 2 was injured heavily, the injury in Group 3 was mild. The differences between Group 1 and Group 2 were not significant. Compared with the rats in Group 1 and Group 2, the ones in Group 3 had more clearly demarcated retina structure and more ordered cells by light microscope and TEM observation. The ONL thicknesses (400 times) of four groups at each time point except between Group 1 and Group 2 were significantly different (P<0.05). The thicknesses of the ONL in Group 1 at three time points were 30.41±4.04µm, 26.11±2.82µm and 20.63±1.06µm, in Group 2 were 31.62±4.54µm, 25.08±3.63µm and 19.07±3.86µm, in Group 3 were 29.75±3.62µm, 28.83±1.97µm and 27.61±1.83µm. In Group 4 the mean of the thickness was 37.35±1.37µm. As time went by, the damage grew more severely. At 24h point, the differences were most significant. Compared with Group 4, the thickness was 46.23% thinner in Group 1, 50.29% thinner in Group 2 and 28.04% thinner in Group 3. The stack structures of membranous disc in Group 3 were injured slightly, but in Group 1 and Group 2 the damage was more obvious by TEM. Compared with Group 4 at each time point, the content of MDA in Group 1 was higher (P<0.05). The content of MDA in Group 3 was significantly lower than those of Group 1 (P<0.05) and Group 2 (P<0.05). Between the Group 1 and Group 2, the MDA concentration at each time point was no significant difference (P>0.05).
Saturated hydrogen saline could protect the retina from light-induced damage by attenuating oxidative stress.
探讨饱和氢盐水对蓝光诱导的大鼠视网膜损伤的影响。
将大鼠暴露于蓝光下6小时诱导视网膜损伤，并在暴露后8小时、16小时和24小时进行检查。100只雌性Sprague-Dawley大鼠随机分为四组。第1组包括30只接受光照且无其他处理的大鼠。第2组包括30只接受光照并腹腔注射生理盐水的大鼠。第3组包括30只接受光照并腹腔注射饱和氢盐水的大鼠。第4组包括另外10只未接受任何处理的大鼠。饱和氢盐水和生理盐水的腹腔注射量按1ml/100g大鼠体重的比例计算。收集标本并进行苏木精-伊红（H-E）染色、超微结构观察、生化检测。通过光学显微镜和透射电子显微镜（TEM）观察形态学变化，并用IPP 6.0测量视网膜外核层（ONL）厚度，同时通过比色法在532nm处测定丙二醛（MDA）含量。
虽然第1组和第2组的视网膜结构严重受损，但第3组的损伤较轻。第1组和第2组之间的差异不显著。通过光学显微镜和TEM观察，与第1组和第2组的大鼠相比，第3组的大鼠视网膜结构分界更清晰，细胞更有序。除第1组和第2组之间外，四个组在每个时间点的ONL厚度（400倍）均有显著差异（P<0.05）。第1组在三个时间点的ONL厚度分别为30.41±4.04μm、26.11±2.82μm和20.63±1.06μm，第2组分别为31.62±4.54μm、25.08±3.63μm和19.07±3.86μm，第3组分别为29.75±3.62μm、28.83±1.97μm和27.61±1.83μm。第4组的平均厚度为37.35±1.37μm。随着时间的推移，损伤变得更加严重。在24小时时，差异最为显著。与第4组相比，第1组的厚度薄46.23%，第2组薄50.29%，第3组薄28.04%。第3组的膜盘堆叠结构损伤轻微，但通过TEM观察，第1组和第2组的损伤更明显。与每个时间点的第4组相比，第1组的MDA含量更高（P<0.05）。第3组的MDA含量显著低于第1组（P<0.05）和第2组（P<0.05）。第1组和第2组之间，每个时间点的MDA浓度无显著差异（P>0.05）。
饱和氢盐水可通过减轻氧化应激保护视网膜免受光诱导损伤。
