Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA.
Am J Trop Med Hyg. 2012 Sep;87(3):565-72. doi: 10.4269/ajtmh.2012.11-0655. Epub 2012 Jul 16.
Serum samples from a total of 71 healthy captive birds belonging to 18 species were collected in July of 2008 in Medellin (Colombia) and tested for flaviviruses. Eighteen of 29 samples from American Flamingoes (Phoenicopterus ruber) were positive for West Nile virus (WNV) by reverse transcription-polymerase chain reaction. Selected positive samples were serially passaged and WNV was confirmed by immunofluorescence. Two isolates (524/08, 9835/08) were characterized in vitro and in vivo. Sequence analysis revealed WNV with 16 nucleotide substitutions resulting in six amino acid changes when compared with the NY99 strain. Colombian (COL) viruses were more closely related to Louisiana isolates from 2001. When compared with attenuated strains isolated from Texas, COL isolates differed in their plaque size and temperature sensitivity phenotype. The COL viruses were pathogenic in embryonated chicken eggs and Balb/c mice.
2008 年 7 月，在哥伦比亚麦德林共采集了 71 只健康圈养鸟类的血清样本，检测其是否感染黄病毒。通过逆转录-聚合酶链反应（RT-PCR）检测，在 29 只美洲火烈鸟（Phoenicopterus ruber）样本中，有 18 份样本呈西尼罗河病毒（WNV）阳性。选择阳性样本进行连续传代，并通过免疫荧光法确认了 WNV。对两个分离株（524/08、9835/08）进行了体外和体内鉴定。序列分析表明，与 NY99 株相比，该病毒有 16 个核苷酸的替换，导致 6 个氨基酸的改变。哥伦比亚（COL）病毒与 2001 年路易斯安那州分离株的亲缘关系更为密切。与从德克萨斯州分离出的减毒株相比，COL 分离株在蚀斑大小和温度敏感性表型上存在差异。COL 病毒在鸡胚和 Balb/c 小鼠中具有致病性。
