Department of Chemical Engineering, University of Washington, Box 351750, Seattle, WA 98195, USA.
Biomaterials. 2012 Nov;33(32):7945-51. doi: 10.1016/j.biomaterials.2012.07.035. Epub 2012 Aug 3.
Surface coatings of high packing densities have been routinely used to prevent nonspecific biomolecular and microorganism attachment. Hydrogels are another class of low fouling materials used to create three-dimensional matrixes for the free diffusion of small analytes or drugs and the high-loading of bio-recognition elements. However, biomolecules are subject to being entrapped within hydrogel matrixes or adhered onto hydrogel surfaces, making them questionable for use in whole blood. Here, we demonstrate the feasibility of a lightly crosslinked poly(carboxybetaine) hydrogel for use in whole blood, as opposite to the conventional wisdom of high packing density in surface coatings. Proteins are able to diffuse in and out of the matrix freely without being altered from their native conformations. In order to demonstrate its long-term performance in whole blood, this hydrogel was used as the surface coating of a glucose sensor. This work paves a new way for the development of surface coatings and sensors to achieve long-term stability and high performance in whole blood.
高填充密度的表面涂层已被常规用于防止非特异性生物分子和微生物附着。水凝胶是另一类低污染材料，用于创建用于小分子分析物或药物自由扩散和生物识别元件高负载的三维基质。然而，生物分子容易被困在水凝胶基质内或附着在水凝胶表面上，这使得它们在全血中使用存在问题。在这里，我们展示了轻度交联的聚（羧基甜菜碱）水凝胶在全血中使用的可行性，与表面涂层中高填充密度的传统观念相反。蛋白质能够自由地扩散进出基质，而不会从其天然构象中改变。为了证明其在全血中的长期性能，该水凝胶被用作葡萄糖传感器的表面涂层。这项工作为表面涂层和传感器的发展开辟了一条新路，以实现全血中的长期稳定性和高性能。
