University of Pittsburgh School of Medicine and UPMC, Heart and Vascular Institute, The Center for Ultrasound Molecular Imaging and Therapeutics, Pittsburgh, Pennsylvania 15261, USA.
J Biomed Opt. 2012 Jul;17(7):070502. doi: 10.1117/1.JBO.17.7.070502.
Several in vitro and in vivo studies have established accelerated thrombolysis using ultrasound (US) induced microbubble (MB) cavitation. However, the mechanisms underlying MB mediated sonothrombolysis are still not completely elucidated. We performed three-dimensional (3-D) volumetric optical coherence tomography (OCT) imaging before and after the application of contrast US to thrombus. The most dramatic reduction in clot volume was observed with US + MB + recombinant tissue plasminogen activator (rt-PA). Thrombus surface erosion in this group on the side of the thrombus exposed to MB and ultrasound was evident on the OCT images. This technique may assist in clarifying the mechanisms underlying sonothrombolysis, especially regarding the importance of US transducer orientation on lytic efficacy and the effects of MB cavitation on thrombus structure.
已有多项体外和体内研究证实，超声（US）诱导微泡（MB）空化可加速溶栓。然而，MB 介导的声溶栓的机制仍不完全清楚。我们在应用对比超声前后对血栓进行了三维（3-D）容积光学相干断层扫描（OCT）成像。在 US + MB + 重组组织纤溶酶原激活剂（rt-PA）组中，观察到血栓体积的最大减少。在 OCT 图像上，在 MB 和超声暴露的血栓一侧，可以明显观察到血栓表面侵蚀。该技术可能有助于阐明声溶栓的机制，特别是关于超声换能器方向对溶栓效果的重要性以及 MB 空化对血栓结构的影响。
