Laboratório de Experimentação Animal e Laboratório de Cardiologia Molecular e Celular, Instituto de Cardiologia/Fundação, Universitária de Cardiologia, Bairro Santana, Porto Alegre, RS, Brazil.
Cardiovasc Diabetol. 2012 Aug 16;11:100. doi: 10.1186/1475-2840-11-100.
Metabolic syndrome is characterized by insulin resistance, which is closely related to GLUT4 content in insulin-sensitive tissues. Thus, we evaluated the GLUT4 expression, insulin resistance and inflammation, characteristics of the metabolic syndrome, in an experimental model.
Spontaneously hypertensive neonate rats (18/group) were treated with monosodium glutamate (MetS) during 9 days, and compared with Wistar-Kyoto (C) and saline-treated SHR (H). Blood pressure (BP) and lipid levels, C-reactive protein (CRP), interleukin 6 (IL-6), TNF-α and adiponectin were evaluated. GLUT4 protein was analysed in the heart, white adipose tissue and gastrocnemius. Studies were performed at 3 (3-mo), 6 (6-mo) and 9 (9-mo) months of age.
MetS rats were more insulin resistant (p<0.001, all ages) and had higher BP (3-mo: p<0.001, 6-mo: p = 0.001, 9-mo: p = 0.015) as compared to C. At 6 months, CRP, IL-6 and TNF-α were higher (p<0.001, all comparisons) in MetS rats vs H, but adiponectin was lower in MetS at 9 months (MetS: 32 ± 2, H: 42 ± 2, C: 45 ± 2 pg/mL; p<0.001). GLUT4 protein was reduced in MetS as compared to C rats at 3, 6 and 9-mo, respectively (Heart: 54%, 50% and 57%; Gastrocnemius: 37%, 56% and 50%; Adipose tissue: 69%, 61% and 69%).
MSG-treated SHR presented all metabolic syndrome characteristics, as well as reduced GLUT4 content, which must play a key role in the impaired glycemic homeostasis of the metabolic syndrome.
代谢综合征的特征是胰岛素抵抗，这与胰岛素敏感组织中的 GLUT4 含量密切相关。因此，我们在实验模型中评估了 GLUT4 表达、胰岛素抵抗和炎症，这些都是代谢综合征的特征。
18 只自发性高血压新生儿大鼠（每组）接受 9 天的谷氨酸单钠（MetS）治疗，并与 Wistar-Kyoto（C）和盐水处理的 SHR（H）进行比较。评估血压（BP）和血脂水平、C 反应蛋白（CRP）、白细胞介素 6（IL-6）、肿瘤坏死因子-α（TNF-α）和脂联素。分析心脏、白色脂肪组织和比目鱼肌中的 GLUT4 蛋白。研究在 3（3 个月）、6（6 个月）和 9（9 个月）个月时进行。
MetS 大鼠的胰岛素抵抗更严重（p<0.001，所有年龄），且血压更高（3 个月：p<0.001，6 个月：p = 0.001，9 个月：p = 0.015）与 C 相比。6 个月时，MetS 大鼠的 CRP、IL-6 和 TNF-α 更高（所有比较 p<0.001），但 MetS 大鼠的脂联素在 9 个月时较低（MetS：32±2，H：42±2，C：45±2pg/mL；p<0.001）。与 C 大鼠相比，MetS 大鼠在 3、6 和 9 个月时的 GLUT4 蛋白分别降低（心脏：54%、50%和 57%；比目鱼肌：37%、56%和 50%；脂肪组织：69%、61%和 69%）。
接受谷氨酸单钠治疗的 SHR 表现出所有代谢综合征特征，以及 GLUT4 含量降低，这在代谢综合征的血糖稳态受损中起着关键作用。
