State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze Water Environment for Ministry of Education, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze Water Environment for Ministry of Education, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
Water Res. 2012 Nov 1;46(17):5509-5516. doi: 10.1016/j.watres.2012.07.025. Epub 2012 Jul 27.
This study examined the impacts of bacteria on the algal biomass, lipid content and efficiency of wastewater treatment during the heterotrophic cultivation of Chlorella pyrenoidosa. Our results showed that soybean-processing wastewater can enhance the accumulation of lipids in algal cells and thus raise the lipid yield in the pure culture. The bacteria coexisting with algae improved the degradation of total nitrogen (TN), total phosphorus (TP), glucose and chemical oxygen demand (COD). Although the biomass productivity of algae was not significantly affected, the total algal lipid content and lipid production rate were slightly reduced when bacteria coexisted with algae. The difference in the compositions of the medium is presumed to be the main contributing factor for the variation in total lipid content in presence and absence of bacteria. The TN, TP, and COD decreased during the assimilatory process undertaken by C. pyrenoidosa, and the removal efficiency of TN by bacteria depended on the type of nitrogen species in the medium. Additionally, the apparent interaction between the bacterial and algal cultures varied with the changes in experimental conditions. Algae could compete with bacteria for the carbon and energy sources, and inhibit the growth of the bacteria in the presence of high organic matter concentration in the medium.
本研究考察了细菌对异养培养过程中栅藻生物质、脂质含量和废水处理效率的影响。结果表明，大豆加工废水可以促进藻类细胞中脂质的积累，从而提高纯培养中的脂质产量。与藻类共存的细菌提高了总氮（TN）、总磷（TP）、葡萄糖和化学需氧量（COD）的降解。尽管藻类的生物量生产力没有受到显著影响，但当细菌与藻类共存时，总藻类脂质含量和脂质生产率略有降低。培养基成分的差异被认为是存在和不存在细菌时总脂质含量变化的主要因素。在栅藻进行同化过程中，TN、TP 和 COD 降低，细菌对 TN 的去除效率取决于培养基中氮物种的类型。此外，细菌和藻类培养物之间的明显相互作用随着实验条件的变化而变化。在培养基中有机物浓度高的情况下，藻类可以与细菌竞争碳源和能源，并抑制细菌的生长。
