Prasad Ram, Katiyar Santosh K
Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL, USA.
Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL, USA; Environmental Health Sciences, University of Alabama at Birmingham, Birmingham, AL, USA; Nutrition Obesity Research Center, School of Public Health, University of Alabama at Birmingham, Birmingham, AL, USA; Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USA; Birmingham Veterans Affairs Medical Center, Birmingham, AL, USA.
Cancer Lett. 2013 Jun 28;334(1):118-26. doi: 10.1016/j.canlet.2012.08.003. Epub 2012 Aug 16.
Here we explore the effect of grape seed proanthocyanidins (GSPs) on pancreatic cancer cell migration and the molecular mechanisms underlying these effects. Treatment of human pancreatic cancer cell lines Miapaca-2, PANC-1 and AsPC-1 with GSPs resulted in inhibition of cell migration (19-82%, P<0.01-0.001), which was associated with decreased phosphorylation of ERK1/2 and inactivation of NF-κB. Treatment of cells with UO126, an inhibitor of MEK, and caffeic acid phenethyl ester, an inhibitor of NF-κB, also inhibited the migration of cells (40-80%, P<0.01-0.001). Inhibition of cell migration by GSPs was associated with reversal of the epithelial-to-mesenchymal transition. This was associated with upregulation of E-cadherin and desmoglein-2 and down-regulation of fibronectin, N-cadherin and vimentin.
在此，我们探讨葡萄籽原花青素（GSPs）对胰腺癌细胞迁移的影响以及这些影响背后的分子机制。用GSPs处理人胰腺癌细胞系Miapaca-2、PANC-1和AsPC-1导致细胞迁移受到抑制（19 - 82%，P<0.01 - 0.001），这与ERK1/2磷酸化减少和NF-κB失活有关。用MEK抑制剂UO126和NF-κB抑制剂咖啡酸苯乙酯处理细胞也抑制了细胞迁移（40 - 80%，P<0.01 - 0.001）。GSPs对细胞迁移的抑制与上皮-间质转化的逆转有关。这与E-钙黏蛋白和桥粒芯糖蛋白-2的上调以及纤连蛋白、N-钙黏蛋白和波形蛋白的下调有关。
