Food Safety & Analytical Quality Control Laboratory, Central Food Technological Research Institute, Mysore 570020, India.
Food Chem Toxicol. 2012 Nov;50(11):4158-62. doi: 10.1016/j.fct.2012.08.034. Epub 2012 Aug 24.
Aflatoxin M(1) (AFM(1)) is a toxic metabolite found in the milk of lactating animals which have consumed feedstuffs contaminated with aflatoxin B(1). Ultra high temperature treated (UHT) milk is a product which is becoming popular in developing countries like India as there is a lack of proper cold storage or refrigeration facilities. In this study, 45 samples of UHT milk of popular brands prevalent in the market were analyzed for the presence of AFM(1) by reversed phase HPLC using fluorescent detector after cleanup of sample with immunoaffinity columns. All samples of plain UHT milk were positive for AFM(1) and 38% of these contained levels more than 0.5 μg/kg, the maximum permitted limit prescribed by the Codex Alimentarius Commission and by the mandatory regulations of the country, the FSSAI Regulations, 2011. In 62.5% of flavored UHT milk, AFM(1) was below detectable levels (0.02 μgL(-1)). However, 12.5% of these samples also contained levels exceeding the maximum permitted limits. AFM(1) was present in 61.6% of the 52 raw milk samples analyzed from the two states of Karnataka and Tamilnadu with a range of 0.1-3.8 μgL(-1). 17.3% of these samples also exceeded the regulatory limits of the country.
黄曲霉毒素 M1（AFM1）是一种在食用了被黄曲霉毒素 B1 污染的饲料的哺乳期动物的奶中发现的有毒代谢物。超高温处理（UHT）奶是一种在印度等发展中国家越来越受欢迎的产品，因为缺乏适当的冷藏或制冷设施。在这项研究中，通过反相高效液相色谱法（HPLC）和荧光检测器，对市场上流行品牌的 45 份 UHT 奶样品进行了分析，在免疫亲和柱净化后检测 AFM1 的存在。所有普通 UHT 奶样品均呈 AFM1 阳性，其中 38%的样品含量超过 0.5μg/kg，这是食品法典委员会和国家强制性法规（FSSAI 法规，2011 年）规定的最大允许限量。在 62.5%的风味 UHT 奶中，AFM1 的含量低于可检测水平（0.02μg/L）。然而，其中 12.5%的样品也含有超过最大允许限量的水平。在来自卡纳塔克邦和泰米尔纳德邦的 52 份生奶样品中，有 61.6%的样品中存在 AFM1，范围为 0.1-3.8μg/L。其中 17.3%的样品也超过了国家的监管限制。
