The Roslin Institute, Easter Bush, Scotland.
Emerg Infect Dis. 2012 Oct;18(10):1574-9. doi: 10.3201/eid1810.120792.
Variant Creutzfeldt-Jakob disease (vCJD) has been reported in 12 countries. We hypothesized that a common strain of agent is responsible for all vCJD cases, regardless of geographic origin. To test this hypothesis, we inoculated strain-typing panels of wild-type mice with brain material from human vCJD case-patients from France, the Netherlands, Italy, and the United States. Mice were assessed for clinical disease, neuropathologic changes, and glycoform profile; results were compared with those for 2 reference vCJD cases from the United Kingdom. Transmission to mice occurred from each sample tested, and data were similar between non-UK and UK cases, with the exception of the ranking of mean clinical incubation times of mouse lines. These findings support the hypothesis that a single strain of infectious agent is responsible for all vCJD infections. However, differences in incubation times require further subpassage in mice to establish any true differences in strain properties between cases.
变异型克雅氏病（vCJD）已在 12 个国家报告过。我们假设，一种共同的病原体株负责所有 vCJD 病例，无论其地理起源如何。为了验证这一假设，我们用来自法国、荷兰、意大利和美国的人类 vCJD 病例患者的脑组织接种了野生型小鼠的菌株分型板。我们评估了小鼠的临床疾病、神经病理学变化和糖型谱；结果与来自英国的 2 个参考 vCJD 病例进行了比较。从每个测试的样本中都可以向小鼠传播，并且非英国和英国病例之间的数据相似，除了小鼠系的平均临床潜伏期的排序外。这些发现支持了一个假设，即一种单一的传染性病原体株负责所有 vCJD 感染。然而，潜伏期的差异需要在小鼠中进一步传代，以确定病例之间在菌株特性上是否存在任何真正的差异。
