Environmental Healthcare Unit, Centre of Biological Sciences, University of Southampton, Southampton, UK.
J Hosp Infect. 2013 Jan;83(1):22-9. doi: 10.1016/j.jhin.2012.08.008. Epub 2012 Oct 23.
The presence and potential build-up of patient material such as proteins in endoscope lumens can have significant implications, including toxic reactions, device damage, inadequate disinfection/sterilization, increased risk of biofilm development and potential transmission of pathogens.
To evaluate potential protein deposition and removal in the channels of flexible luminal endoscopes during a simple contamination/cleaning cycle.
The level of contamination present on disposable endoscopy forceps which come into contact with the lumen of biopsy channels was evaluated. Following observations in endoscopy units, factors influencing protein adsorption inside luminal endoscope channels and the action of current initial cleaning techniques were evaluated using a proteinaceous test soil and very sensitive fluorescence epimicroscopy.
Disposable endoscope accessories appear to be likely to contribute to the contamination of lumens, and were useful indicators of the amount of proteinaceous soil transiting through the channels of luminal endoscopes. Enzymatic cleaning according to the manufacturer's recommendations and brushing of the channels were ineffective at removing all proteinaceous residues from new endoscope channels after a single contamination. Rinsing immediately after contamination only led to a slight improvement in decontamination outcome.
Limited action of current decontamination procedures and the lack of applicable quality control methods to assess the cleanliness of channels between patients contribute to increasing the risk of cross-infection of potentially harmful micro-organisms and molecules during endoscopy procedures.
内镜通道内存在患者材料(如蛋白质)会产生严重影响,包括毒性反应、器械损坏、消毒/灭菌不充分、生物膜形成风险增加以及病原体潜在传播。
评估简易污染/清洗周期中柔性内腔内镜通道内潜在的蛋白质沉积和清除情况。
评估接触活检通道内腔的一次性内镜活检钳上的污染程度。在观察内镜使用单元后,使用蛋白质测试土和非常敏感的荧光相差显微镜,评估影响内腔内镜通道内蛋白质吸附的因素和当前初始清洗技术的作用。
一次性内镜附件似乎容易导致内腔污染,是内腔内镜通道内蛋白质污染量的有用指示物。按照制造商的建议进行酶清洗和刷洗通道后,在单次污染后,新的内镜通道内仍无法彻底清除所有蛋白质残留。污染后立即冲洗仅略微改善去污效果。
目前的去污程序作用有限,缺乏适用于评估患者间通道清洁度的质量控制方法,这增加了内镜检查过程中潜在有害微生物和分子交叉感染的风险。