Retter M, Middleton P J, Tam J S, Petric M
J Clin Microbiol. 1979 Oct;10(4):574-8. doi: 10.1128/jcm.10.4.574-578.1979.
Adenoviruses can be demonstrated readily in the stools of pediatric gastroenteritis patients by electron microscopy or counterimmunoelectrophoresis, but in 45% of these cases the virus will not grow in cell culture. Indirect immunofluorescence microscopy can be used to detect nongrowing strains of adenovirus; these strains have a unique single-cell fluorescence pattern. Hematoxylin and eosin staining reveals adenovirus-like inclusion bodies in the same distribution as fluorescent cells. Pretreatment of nongrowing adenovirus with convalescent-phase patient serum neutralized its ability to infect the cell culture and produce fluorescent cells. Indirect immunofluorescence microscopy compared favorably with electron microscopy in demonstrating virus in the stools of patients.
通过电子显微镜或对流免疫电泳可很容易地在小儿肠胃炎患者的粪便中检测到腺病毒，但在这些病例中，45%的病毒无法在细胞培养中生长。间接免疫荧光显微镜可用于检测腺病毒的非生长菌株；这些菌株具有独特的单细胞荧光模式。苏木精和伊红染色显示腺病毒样包涵体的分布与荧光细胞相同。用恢复期患者血清预处理非生长型腺病毒可中和其感染细胞培养物并产生荧光细胞的能力。在检测患者粪便中的病毒时，间接免疫荧光显微镜与电子显微镜相比效果良好。
