Auclair C, Gouyette A, Levy A, Emerit I
Institut Gustave Roussy Villejuif, Paris, CNRS, France.
Arch Biochem Biophys. 1990 Apr;278(1):238-44. doi: 10.1016/0003-9861(90)90253-u.
In the present study, we attempted to identify the chemical nature of the clastogenic factor (CF) from patients with progressive systemic sclerosis (scleroderma). Computerized mass spectrometry of clastogenic fractions obtained by HPLC of plasma ultrafiltrates detected molecular peaks compatible with inosine triphosphate and inosine diphosphate (ITP and IDP). The concomitant detection of IDP, together with ITP, and the absence of these peaks in nonclastogenic fractions and corresponding control fractions are arguments in favor of a biological relevance of these observations. The most important confirmation came from the clastogenic effect of commercial ITP and IDP added to the culture medium of the test cultures. The induction of chromatid type damage by these substances in lymphocytes exposed in the G0 phase of their cell cycle and the prevention of this damage by superoxide dismutase are analogous to the observations with CF.
在本研究中，我们试图鉴定进行性系统性硬化症（硬皮病）患者的致断裂因子（CF）的化学性质。通过血浆超滤物的高效液相色谱法获得的致断裂组分的计算机化质谱检测到与三磷酸肌苷和二磷酸肌苷（ITP和IDP）相符的分子峰。IDP与ITP的同时检测，以及在非致断裂组分和相应对照组分中不存在这些峰，支持了这些观察结果具有生物学相关性。最重要的证实来自添加到测试培养物培养基中的商业ITP和IDP的致断裂作用。这些物质在细胞周期G0期暴露的淋巴细胞中诱导染色单体型损伤，以及超氧化物歧化酶对这种损伤的预防作用，与CF的观察结果相似。
