Asgary Sedigheh, Rahimi Parivash, Mahzouni Parvin, Madani Hossein
Department of Basic Sciences, Isfahan Cardiovascular Research Center, Isfahan Cardiovascular Research InstituteApplied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
J Res Med Sci. 2012 Apr;17(4):386-92.
Carthamus tinctorius L. (Compositae) has been used in Iranian traditional medicine for treatment of diabetes. In this study, anti-diabetic effect of its hydroalcoholic extract was compared with that of glibenclamide.
MALE WHITE WISTAR RATS WERE RANDOMLY ALLOCATED INTO FOUR GROUPS OF SIX EACH: nondiabetic control; diabetic control; diabetic treated with hydroalcoholic extract of Carthamus tinctorius (200 mg kg(-1) BW); diabetic rats treated with glibenclamide (0.6 mg kg(-1) BW). Alloxan was administered (120 mg kg(-1) BW), intraperitoneally to induce diabetes. Fasting blood samples were collected three times, before injection of alloxan, two weeks and six weeks after injection of alloxan and fasting blood sugar (FBS), Hb A1C, insulin, cholesterol, LDL-C, HDL-C, VLDL-C, triglyceride, alkaline phosphatase (ALP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured each time.
FBS, triglyceride, cholesterol, LDL-C and VLDL-C had a meaningful decrease in diabetic rats treated with Carthamus tinctorius and diabetic rats treated with glibenclamide as compared with diabetic rats with no treatment. Insulin level increased significantly in diabetic groups received treatment (glibenclamide or Carthamus tinctorius L) in comparison with diabetic group with no treatment. The histological study revealed size of islets of Langerhans enlarged significantly consequentially as compared with diabetic rats with no treatment. The extract appeared non toxic as evidenced by normal levels of AST, ALP and ALT. Effects of administrating glibenclamide or extract of Carthamus tinctorius L on all biochemical parameters discussed above showed no difference and both tend to bring the values to near normal.
These results suggested that the hydroalcoholic extract of Carthamus tinctorius possesses beneficial effect on treatment of diabetes.
红花（菊科）在伊朗传统医学中用于治疗糖尿病。在本研究中，将其水醇提取物的抗糖尿病作用与格列本脲进行了比较。
雄性白色Wistar大鼠随机分为四组，每组六只：非糖尿病对照组；糖尿病对照组；用红花水醇提取物（200mg/kg体重）治疗的糖尿病组；用格列本脲（0.6mg/kg体重）治疗的糖尿病大鼠。腹腔注射四氧嘧啶（120mg/kg体重）诱导糖尿病。在注射四氧嘧啶前、注射四氧嘧啶后两周和六周采集三次空腹血样，每次测量空腹血糖（FBS）、糖化血红蛋白（Hb A1C）、胰岛素、胆固醇、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）、极低密度脂蛋白胆固醇（VLDL-C）、甘油三酯、碱性磷酸酶（ALP）、丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）。
与未治疗的糖尿病大鼠相比，用红花治疗的糖尿病大鼠和用格列本脲治疗的糖尿病大鼠的FBS、甘油三酯、胆固醇、LDL-C和VLDL-C有显著降低。与未治疗的糖尿病组相比，接受治疗（格列本脲或红花）的糖尿病组胰岛素水平显著升高。组织学研究显示，与未治疗的糖尿病大鼠相比，胰岛大小显著增大。AST、ALP和ALT水平正常证明该提取物无毒。格列本脲或红花提取物对上述所有生化参数的影响无差异，两者都倾向于使数值接近正常。
这些结果表明，红花水醇提取物对糖尿病治疗具有有益作用。
