Laboratory of Anatomy and Physiology of Farm Animals, Department of Animal Science, Agricultural University of Athens (AUA), Iera Odos 75 Str., 11855 Athens, Greece.
Department of Swine Diseases, National Veterinary Research Institute, Partyzantów Avenue 57, 24-100 Puławy, Poland.
Viruses. 2022 May 7;14(5):988. doi: 10.3390/v14050988.
Swine viral diseases challenge the sector's sustainability by affecting productivity and the health and welfare of the animals. The lack of antiviral drugs and/or effective vaccines renders early and reliable diagnosis the basis of viral disease management, underlining the importance of point-of-care (POC) diagnostics. A novel POC diagnostic device utilizing photonic integrated circuits (PICs), microfluidics, and information and communication technologies for the detection of porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza A (SIV) was validated using spiked and clinical oral fluid samples. Metrics including sensitivity, specificity, accuracy, precision, positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR) were calculated to assess the performance of the device. For PRRSV, the device achieved a sensitivity of 83.5%, specificity of 77.8%, and DOR values of 17.66, whereas the values for SIV were 81.8%, 82.2%, and 20.81, respectively. The POC device and PICs can be used for the detection of PRRSV and SIV in the field, paving the way for the introduction of novel technologies in the field of animal POC diagnostics to further optimize livestock biosecurity.
猪病毒性疾病通过影响生产力以及动物的健康和福利，对该行业的可持续性构成挑战。由于缺乏抗病毒药物和/或有效的疫苗，早期和可靠的诊断成为病毒性疾病管理的基础，这突显了即时检测（POC）诊断的重要性。一种利用光子集成电路（PICs）、微流控技术以及信息和通信技术来检测猪繁殖与呼吸综合征病毒（PRRSV）和猪流感 A 型病毒（SIV）的新型 POC 诊断设备，已通过添加和临床口腔液样本进行了验证。通过计算敏感性、特异性、准确性、精密度、阳性似然比（PLR）、阴性似然比（NLR）和诊断比值比（DOR）等指标来评估该设备的性能。对于 PRRSV，该设备的灵敏度为 83.5%，特异性为 77.8%，DOR 值为 17.66；而对于 SIV，其灵敏度为 81.8%，特异性为 82.2%，DOR 值为 20.81。该 POC 设备和 PICs 可用于现场检测 PRRSV 和 SIV，为在动物 POC 诊断领域引入新型技术铺平道路，以进一步优化牲畜生物安全。
