Department of Biology, The College at Brockport, State University of New York , Brockport, NY 14420, USA.
Zebrafish. 2013 Jun;10(2):154-60. doi: 10.1089/zeb.2012.0766. Epub 2013 Jan 8.
Interstitial cells of Cajal (ICC) provide a pacemaker signal for coordinated motility patterns in the mammalian gastrointestinal (GI) tract. Kit signaling is required for development and maintenance of ICC, and these cells can be identified by Kit-like immunoreactivity. The zebrafish GI tract has two distinct ICC networks similar to mammals, suggesting a similar role in the generation of GI motility; however, a functional role for Kit-positive cells in zebrafish has not been determined. Analysis of GI motility in intact zebrafish larvae was performed during development and after disruption of Kit signaling. Development of coordinated motility patterns occurred after 5 days post-fertilization (dpf) and correlated with appearance of Kit-positive cells. Disruptions of Kit signaling using the Kit antagonist imatinib mesylate, and in Sparse, a null kita mutant, also disrupted development of coordinated motility patterns. These data suggest that Kit signaling is necessary for development of coordinated motility patterns and that Kit-positive cells in zebrafish are necessary for coordinated motility patterns.
Cajal 间质细胞 (ICC) 为哺乳动物胃肠道 (GI) 运动的协调模式提供起搏信号。Kit 信号对于 ICC 的发育和维持是必需的，这些细胞可以通过 Kit 样免疫反应来识别。斑马鱼的胃肠道有两个类似于哺乳动物的独特 ICC 网络，这表明它们在产生 GI 运动方面具有相似的作用；然而，Kit 阳性细胞在斑马鱼中的功能作用尚未确定。在发育过程中和 Kit 信号中断后，对完整斑马鱼幼虫的 GI 运动进行了分析。在受精后 5 天（dpf）后出现协调运动模式，并与 Kit 阳性细胞的出现相关。使用 Kit 拮抗剂伊马替尼和 Sparse（一种 null kita 突变体）中断 Kit 信号也会破坏协调运动模式的发育。这些数据表明，Kit 信号对于协调运动模式的发育是必需的，并且斑马鱼中的 Kit 阳性细胞对于协调运动模式是必需的。
