Sabitha Vijayakumar, Ramachandran Subramaniam, Naveen Koikaramparambil Robert, Panneerselvam Kaliyamoorthy
Department of Bioinformatics, Karunya University, Coimbatore, India.
J Ayurveda Integr Med. 2012 Oct;3(4):188-93. doi: 10.4103/0975-9476.104432.
Abelmoschus esculentus (L.) Moench. fruit is a commonly consumed vegetable in many countries due to its rich medicinal value. However, till date, in vivo antioxidant property of A. esculentus has not been scientifically documented in animal models.
The present investigation was aimed to evaluate the in vivo antioxidant property of A. esculentus (L.) Moench. peel and seed powder (AEPP and AESP) in streptozotocin (STZ)-induced diabetic rats.
In rats, acute toxicity assessment of AEPP and AESP at 2 g/kg did not show any toxicity. Diabetes was induced by STZ (60 mg/kg, i.p.) injection and diabetic rats received AEPP (100 and 200 mg/kg) as well as AESP (100 and 200 mg/ kg) orally up to 28 days. At the end of the 28 day, diabetic rats were killed and liver, kidney and pancreas were collected to determine superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), and lipid peroxidation level.
In diabetic rats, significant (P < 0.001) reduction of liver, kidney and pancreas SOD, CAT, GPx, GSH levels and increase in thiobarbituric acid reactive substances (TBARS) were observed as compared to normal control rats. Administration of both doses of AEPP and AESP significantly (P < 0.001 and P < 0.01) increased liver, kidney and pancreas SOD, CAT, GPx, GSH levels and decreased TBARS (P < 0.001) levels in diabetic rats compared to diabetic control rats.
Our findings confirmed that A. esculentus peel and seed powder has significant in vivo antioxidant property in diabetic rats.
由于具有丰富的药用价值，黄秋葵果实是许多国家常见的食用蔬菜。然而，迄今为止，黄秋葵的体内抗氧化特性尚未在动物模型中得到科学记录。
本研究旨在评估黄秋葵果皮和种子粉（AEPP和AESP）对链脲佐菌素（STZ）诱导的糖尿病大鼠的体内抗氧化特性。
在大鼠中，2 g/kg剂量的AEPP和AESP急性毒性评估未显示任何毒性。通过腹腔注射STZ（60 mg/kg）诱导糖尿病，糖尿病大鼠口服AEPP（100和200 mg/kg）以及AESP（100和200 mg/kg），持续28天。在第28天结束时，处死糖尿病大鼠，收集肝脏、肾脏和胰腺以测定超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、还原型谷胱甘肽（GSH）和脂质过氧化水平。
与正常对照大鼠相比，糖尿病大鼠肝脏、肾脏和胰腺的SOD、CAT、GPx、GSH水平显著降低（P < 0.001），硫代巴比妥酸反应性物质（TBARS）水平升高。与糖尿病对照大鼠相比，给予两种剂量的AEPP和AESP均显著提高了糖尿病大鼠肝脏、肾脏和胰腺的SOD、CAT、GPx、GSH水平，并降低了TBARS水平（P < 0.001）。
我们的研究结果证实，黄秋葵果皮和种子粉对糖尿病大鼠具有显著的体内抗氧化特性。
