Gaspar Diana Alves, Gomide Viviane, Monteiro Fernando Jorge
Departamento de Engenharia Metalúrgica e de Materiais, Universidade do Porto, Faculdade de Engenharia (FEUP), Porto, Portugal.
Biomatter. 2012 Oct-Dec;2(4):167-75. doi: 10.4161/biom.22170.
Tissue engineering has emerged as a possible alternative to current treatments for bone injuries and defects. However, the common tissue engineering approach presents some obstacles to the development of functional tissues, such as insufficient nutrient and metabolite transport and non-homogenous cell distribution. Culture of bone cells in three-dimensional constructs in bioreactor systems is a solution for those problems as it improves mass transport in the culture system. For bone tissue engineering spinner flasks, rotating wall vessels and perfusion systems have been investigated, and based on these, variations that support cell seeding and mechanical stimulation have also been researched. This review aims at providing an overview of the concepts, advantages and future applications of bioreactor systems for bone tissue engineering with emphasis on the design of different perfusion systems and parameters that can be optimized.
组织工程学已成为当前治疗骨损伤和骨缺损的一种可能替代方法。然而，常见的组织工程方法在功能性组织的发育方面存在一些障碍，比如营养物质和代谢产物运输不足以及细胞分布不均一。在生物反应器系统的三维构建物中培养骨细胞是解决这些问题的一种方法，因为它改善了培养系统中的物质运输。对于骨组织工程旋转瓶、旋转壁式生物反应器和灌注系统已进行了研究，基于这些，还研究了支持细胞接种和机械刺激的变体。本综述旨在概述用于骨组织工程的生物反应器系统的概念、优势和未来应用，重点关注不同灌注系统的设计以及可优化的参数。
