ANSES, Ploufragan-Plouzané Laboratory, BP 53, 22440 Ploufragan, France.
Toxins (Basel). 2013 Apr 23;5(4):841-64. doi: 10.3390/toxins5040841.
The objective of this study was to measure the effects of chronic exposure to fumonisins via the ingestion of feed containing naturally contaminated corn in growing pigs infected or not with Salmonella spp. This exposure to a moderate dietary concentration of fumonisins (11.8 ppm) was sufficient to induce a biological effect in pigs (Sa/So ratio), but no mortality or pathology was observed over 63 days of exposure. No mortality or related clinical signs, even in cases of inoculation with Salmonella (5 × 10⁴ CFU), were observed either. Fumonisins, at these concentrations, did not affect the ability of lymphocytes to proliferate in the presence of mitogens, but after seven days post-inoculation they led to inhibition of the ability of specific Salmonella lymphocytes to proliferate following exposure to a specific Salmonella antigen. However, the ingestion of fumonisins had no impact on Salmonella translocation or seroconversion in inoculated pigs. The inoculation of Salmonella did not affect faecal microbiota profiles, but exposure to moderate concentrations of fumonisins transiently affected the digestive microbiota balance. In cases of co-infection with fumonisins and Salmonella, the microbiota profiles were rapidly and clearly modified as early as 48 h post-Salmonella inoculation. Therefore under these experimental conditions, exposure to an average concentration of fumonisins in naturally contaminated feed had no effect on pig health but did affect the digestive microbiota balance, with Salmonella exposure amplifying this phenomenon.
本研究的目的是通过摄入含有自然污染玉米的饲料来测量生长猪在感染或未感染沙门氏菌的情况下慢性暴露于伏马菌素的影响。这种中等膳食浓度（11.8ppm）的伏马菌素暴露足以对猪产生生物学效应（Sa/So 比值），但在 63 天的暴露期间没有观察到死亡率或病理学变化。即使在接种沙门氏菌（5×10⁴CFU）的情况下，也没有观察到死亡率或相关临床症状。在这些浓度下，伏马菌素不影响淋巴细胞在有丝分裂原存在的情况下增殖的能力，但在接种后七天，它们导致对特定沙门氏菌抗原暴露后特定沙门氏菌淋巴细胞增殖能力的抑制。然而，摄入伏马菌素不会影响接种猪的沙门氏菌易位或血清转换。沙门氏菌的接种不会影响粪便微生物群的图谱，但暴露于中等浓度的伏马菌素会暂时影响消化微生物群的平衡。在伏马菌素和沙门氏菌共同感染的情况下，早在沙门氏菌接种后 48 小时，微生物群的图谱就迅速且明显地发生了改变。因此，在这些实验条件下，摄入天然污染饲料中的平均伏马菌素浓度对猪的健康没有影响，但会影响消化微生物群的平衡，而沙门氏菌的暴露会放大这种现象。
