Bibi Y, Nisa S, Zia M, Waheed A, Ahmed S, Chaudhary M F
Department of Botany, PMAS University of Arid Agriculture, Rawalpindi, Pakistan.
Indian J Pharm Sci. 2012 Jul;74(4):375-9. doi: 10.4103/0250-474X.107085.
Pistacia integerrima Stew. ex Brand (Anacardiaceae) is an ethanobotanically important plant species traditionally used in the treatment of chronic wounds, jaundice, dysentery, etc. The crude extract from Pistacia integerrima and its fractions were tested for cytotoxic activity against Michigan Cancer Foundation-7 human breast cancer cell line. We have also investigated that crude stem extract of this plant also exhibits the antitumour as well as antifungal potential activities. Moreover, we have also studied that the crude extract inhibited Michigan Cancer Foundation-7 cell viability in a dose-dependent manner; the poor toxicity (1.6%) at 10 μg/ml to moderate toxicity (55.4%) at 100 μg/ml. The IC50 values calculated were 90.9 μg/ml. The ethyl acetate and chloroform fractions at a concentration of 200 μg/ml showed ~100 and 97.4% inhibition against Michigan Cancer Foundation-7 cell line, respectively. The crude methanol extract also showed good antitumour (IC50 125 ppm) activity, but weak antifungal activity. These findings reveal that the ethyl acetate and chloroform fractions of Pistacia integerrima are potent cytotoxic fractions, and could be an alternate candidate for the development of novel biologically active compounds.
全缘黄连木（Pistacia integerrima Stew. ex Brand，漆树科）是一种在民族植物学上具有重要意义的植物物种，传统上用于治疗慢性伤口、黄疸、痢疾等疾病。对全缘黄连木的粗提物及其馏分进行了针对密歇根癌症基金会-7人乳腺癌细胞系的细胞毒性活性测试。我们还研究了该植物的粗茎提取物也具有抗肿瘤以及抗真菌的潜在活性。此外，我们还研究发现粗提物以剂量依赖的方式抑制密歇根癌症基金会-7细胞的活力；在10μg/ml时毒性较低（1.6%），在100μg/ml时毒性为中等（55.4%）。计算得出的IC50值为90.9μg/ml。浓度为200μg/ml的乙酸乙酯和氯仿馏分分别对密歇根癌症基金会-7细胞系显示出约100%和97.4%的抑制率。粗甲醇提取物也显示出良好的抗肿瘤活性（IC50为125ppm），但抗真菌活性较弱。这些发现表明，全缘黄连木的乙酸乙酯和氯仿馏分是有效的细胞毒性馏分，可能是开发新型生物活性化合物的替代候选物。
