National Cancer Institute, Bethesda, MD 20892, USA.
Science. 2013 Aug 9;341(6146):660-4. doi: 10.1126/science.1237150.
Chromosome translocations are a hallmark of cancer cells. We have developed an experimental system to visualize the formation of translocations in living cells and apply it to characterize the spatial and dynamic properties of translocation formation. We demonstrate that translocations form within hours of the occurrence of double-strand breaks (DSBs) and that their formation is cell cycle-independent. Translocations form preferentially between prepositioned genome elements, and perturbation of key factors of the DNA repair machinery uncouples DSB pairing from translocation formation. These observations generate a spatiotemporal framework for the formation of translocations in living cells.
染色体易位是癌细胞的一个标志。我们开发了一种实验系统，可以在活细胞中观察易位的形成，并应用该系统来描述易位形成的空间和动态特性。我们证明，易位在双链断裂 (DSB) 发生后的数小时内形成，并且其形成与细胞周期无关。易位优先形成于预先定位的基因组元件之间，并且 DNA 修复机制的关键因素的扰动将 DSB 配对与易位形成解耦。这些观察结果为活细胞中易位的形成提供了一个时空框架。
