Dept. of Biomolecular Chemistry, Leibniz Institute for Natural Product Research and Infection Biology, HKI, Beutenbergstrasse 11a, 07745 Jena (Germany) http://www.hki-jena.de.
Angew Chem Int Ed Engl. 2013 Sep 27;52(40):10564-8. doi: 10.1002/anie.201305595. Epub 2013 Aug 22.
Sulfur for fire: The molecular basis for the biosynthesis of the antimetabolite 6-thioguanine (6TG) was unveiled in Erwinia amylovora, the causative agent of fire blight. Bioinformatics, heterologous pathway reconstitution in E. coli, and mutational analyses indicate that the protein YcfA mediates guanine thionation in analogy to 2-thiouridylase. Assays in planta and in cell cultures reveal for the first time a crucial role of 6TG in fire blight pathogenesis.
在导致火疫病的病原体——果胶菌（Erwinia amylovora）中，揭示了抗代谢物 6-硫代鸟嘌呤（6TG）生物合成的分子基础。生物信息学、大肠杆菌中的异源途径重建和突变分析表明，蛋白质 YcfA 介导鸟嘌呤硫代类似于 2-硫尿苷酶。在植物体内和细胞培养物中的测定首次揭示了 6TG 在火疫病发病机制中的关键作用。
