Xu Gaofeng, Wang Maode, Xie Wanfu, Bai Xiaobin
Department of Neurosurgery, The First Affiliated Hospital of Medical School, Xi'an Jiaotong University, 277 Yanta West Road, Xi'an, 710061, China,
Tumour Biol. 2014 Feb;35(2):1389-95. doi: 10.1007/s13277-013-1191-3. Epub 2013 Sep 19.
Three polymorphisms of X-ray repair cross-complementing groups 1 (XRCC1) Arg194Trp, Arg280His, and Arg399Gln may be associated with the individual susceptibility to glioma. The aim of this study was to investigate any association between three polymorphisms of the XRCC1 gene at codon 194, 280, and 399 and potential glioma risk. We conducted a hospital-based case-control study in northwest China. A total of 1,772 subjects, including 886 glioma patients and 886 healthy controls, were recruited in this study. The peripheral blood samples were extracted. Polymerase chain reaction-restriction fragment length polymorphism method was used to test genotypes. Glioma patients had a significantly higher frequency of XRCC1 194 TT (odds ratio [OR] = 1.76, 95 % confidence interval [CI] = 1.14, 2.72; P = 0.01) and XRCC1 399 AA genotype (OR = 1.62, 95 % CI = 1.09, 2.40; P = 0.02) than controls. When stratified by the grade of glioma, patients with WHO IV glioma had a significantly higher frequency of XRCC1 194 TT (OR = 1.60, 95 % CI = 1.02, 2.51; P = 0.04) and XRCC1 399 AA genotype (OR = 1.59, 95 % CI = 1.04, 2.42; P = 0.03). When stratified by the histology of glioma, there was no significant difference in the distribution of each genotype. This study suggested that XRCC1 Arg194Trp and Arg399Gln polymorphisms were associated with the risk of glioma.
X射线修复交叉互补基因1（XRCC1）的三种多态性，即精氨酸194色氨酸（Arg194Trp）、精氨酸280组氨酸（Arg280His）和精氨酸399谷氨酰胺（Arg399Gln），可能与个体患神经胶质瘤的易感性相关。本研究的目的是调查XRCC1基因第194、280和399密码子的三种多态性与潜在神经胶质瘤风险之间的任何关联。我们在中国西北部进行了一项基于医院的病例对照研究。本研究共招募了1772名受试者，包括886名神经胶质瘤患者和886名健康对照。采集外周血样本。采用聚合酶链反应-限制性片段长度多态性方法检测基因型。神经胶质瘤患者中XRCC1 194 TT基因型（比值比[OR]=1.76，95%置信区间[CI]=1.14，2.72；P=0.01）和XRCC1 399 AA基因型（OR=1.62，95%CI=1.09，2.40；P=0.02）的频率显著高于对照组。按神经胶质瘤分级分层时，世界卫生组织IV级神经胶质瘤患者中XRCC1 194 TT基因型（OR=1.60，95%CI=1.02，2.51；P=0.04）和XRCC1 399 AA基因型（OR=1.59，95%CI=1.04，2.42；P=0.03）的频率显著更高。按神经胶质瘤组织学分层时，各基因型分布无显著差异。本研究表明，XRCC1 Arg194Trp和Arg399Gln多态性与神经胶质瘤风险相关。
