用于培养体液的隔离器系统和大容量离心法的评估
Evaluation of isolator system and large-volume centrifugation method for culturing body fluids.
作者信息
Elston H R, Wang M, Philip A
机构信息
Clinical Microbiology Laboratory, Veterans Administration Hospital, Loma Linda, California 92357.
出版信息
J Clin Microbiol. 1990 Jan;28(1):124-5. doi: 10.1128/jcm.28.1.124-125.1990.
Abstract
The Isolator system was compared with the large-volume centrifugation method for processing and recovering organisms from body fluids other than blood, cerebrospinal fluid, and urine. A total of 155 body fluid samples were processed for the recovery of clinically significant organisms. Of the 55 positive cultures, Isolator detected 94% and the large-volume centrifugation method detected 64%. The time necessary to indicate positivity was not significantly different in the two methods; however, in five cases, the Isolator system yielded clinically significant organisms 24 h sooner than the conventional method. The Isolator system was found to be a more sensitive alternative than the conventional large-volume centrifugation method.
摘要
将隔离器系统与大容量离心法进行了比较，以从血液、脑脊液和尿液以外的体液中处理和回收微生物。总共对155份体液样本进行了处理，以回收具有临床意义的微生物。在55份阳性培养物中，隔离器检测出94%，大容量离心法检测出64%。两种方法显示阳性所需的时间没有显著差异；然而，在5例病例中，隔离器系统比传统方法早24小时分离出具有临床意义的微生物。结果发现，与传统的大容量离心法相比，隔离器系统是一种更敏感的替代方法。
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