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用于培养体液的隔离器系统和大容量离心法的评估

Evaluation of isolator system and large-volume centrifugation method for culturing body fluids.

作者信息

Elston H R, Wang M, Philip A

机构信息

Clinical Microbiology Laboratory, Veterans Administration Hospital, Loma Linda, California 92357.

出版信息

J Clin Microbiol. 1990 Jan;28(1):124-5. doi: 10.1128/jcm.28.1.124-125.1990.

DOI:10.1128/jcm.28.1.124-125.1990
PMID:2405006
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC269550/
Abstract

The Isolator system was compared with the large-volume centrifugation method for processing and recovering organisms from body fluids other than blood, cerebrospinal fluid, and urine. A total of 155 body fluid samples were processed for the recovery of clinically significant organisms. Of the 55 positive cultures, Isolator detected 94% and the large-volume centrifugation method detected 64%. The time necessary to indicate positivity was not significantly different in the two methods; however, in five cases, the Isolator system yielded clinically significant organisms 24 h sooner than the conventional method. The Isolator system was found to be a more sensitive alternative than the conventional large-volume centrifugation method.

摘要

将隔离器系统与大容量离心法进行了比较,以从血液、脑脊液和尿液以外的体液中处理和回收微生物。总共对155份体液样本进行了处理,以回收具有临床意义的微生物。在55份阳性培养物中,隔离器检测出94%,大容量离心法检测出64%。两种方法显示阳性所需的时间没有显著差异;然而,在5例病例中,隔离器系统比传统方法早24小时分离出具有临床意义的微生物。结果发现,与传统的大容量离心法相比,隔离器系统是一种更敏感的替代方法。

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本文引用的文献

1
Peritonitis during continuous ambulatory peritoneal dialysis.持续性非卧床腹膜透析期间的腹膜炎
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Comparison of methods for processing dialysate in suspected continuous ambulatory peritoneal dialysis-associated peritonitis.疑似持续性非卧床腹膜透析相关性腹膜炎时透析液处理方法的比较
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6
Improved method for recovery of peritonitis-causing microorganisms from peritoneal dialysate.从腹膜透析液中回收引起腹膜炎的微生物的改良方法。
J Clin Microbiol. 1987 Feb;25(2):383-4. doi: 10.1128/jcm.25.2.383-384.1987.
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Inoculation of blood culture bottles with ascitic fluid. Improved detection of spontaneous bacterial peritonitis.用腹水接种血培养瓶。提高自发性细菌性腹膜炎的检测率。
Arch Intern Med. 1987 Jan;147(1):73-5.
8
Laboratory diagnosis of peritonitis in continuous ambulatory peritoneal dialysis by lysis and centrifugation.通过裂解和离心进行持续性非卧床腹膜透析中腹膜炎的实验室诊断。
J Clin Pathol. 1986 Aug;39(8):925-6. doi: 10.1136/jcp.39.8.925.
9
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J Clin Microbiol. 1986 Feb;23(2):350-3. doi: 10.1128/jcm.23.2.350-353.1986.
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