中国人群中血管紧张素转换酶（ACE）基因插入/缺失多态性、ACE活性及ACE mRNA表达与高血压的相关性
Associations of ACE gene insertion/deletion polymorphism, ACE activity, and ACE mRNA expression with hypertension in a Chinese population.
作者信息
He Qingfang, Fan Chunhong, Yu Min, Wallar Gina, Zhang Zuo-Feng, Wang Lixin, Zhang Xinwei, Hu Ruying
机构信息
Zhejiang Provincial Center for Disease Prevention and Control, Hangzhou, China.
出版信息
PLoS One. 2013 Oct 1;8(10):e75870. doi: 10.1371/journal.pone.0075870. eCollection 2013.
BACKGROUND
The present study was designed to explore the association of angiotensin converting enzyme (ACE) gene insertion/deletion (I/D, rs4646994) polymorphism, plasma ACE activity, and circulating ACE mRNA expression with essential hypertension (EH) in a Chinese population. In addition, a new detection method for circulating ACE mRNA expression was explored.
METHODS
The research was approved by the ethics committee of Zhejiang Provincial Center for Disease Prevention and Control. Written informed consent was obtained prior to the investigation. 221 hypertensives (cases) and 221 normotensives (controls) were interviewed, subjected to a physical examination, and provided blood for biochemical and genetic tests. The ACE mRNA expression was analyzed by real time fluorescent quantitative Reverse Transcription PCR (FQ-RT-PCR). We performed logistic regression to assess associations of ACE I/D genotypes, ACE activity, and ACE mRNA expression levels with hypertension.
RESULTS
The results of the multivariate logistic regression analysis showed that the additive model (ID, DD versus II) of the ACE genotype revealed an association with hypertension with adjusted OR of 1.43(95% CI: 1.04-1.97), and ACE ID genotype with adjusted OR of 1.72(95% CI: 1.01-2.92), DD genotype with adjusted OR of 1.94(95% CI: 1.01-3.73), respectively. In addition, our data also indicate that plasma ACE activity (adjusted OR was 1.13(95% CI: 1.08-1.18)) was significantly related to hypertension. However, the plasma ACE mRNA expressions were not different between the cases and controls.
CONCLUSION
ACE I/D polymorphism and ACE activity revealed significant influence on hypertension, while circulating ACE mRNA expression was not important factors associated with hypertension in this Chinese population. The detection of circulating ACE mRNA expression by FQ-RT-PCR might be a useful method for early screening and monitoring of EH.
背景
本研究旨在探讨血管紧张素转换酶（ACE）基因插入/缺失（I/D，rs4646994）多态性、血浆ACE活性及循环ACE mRNA表达与中国人群原发性高血压（EH）的相关性。此外，还探索了一种循环ACE mRNA表达的新检测方法。
方法
本研究经浙江省疾病预防控制中心伦理委员会批准。调查前获得书面知情同意书。对221例高血压患者（病例组）和221例血压正常者（对照组）进行访谈、体格检查，并采集血液进行生化和基因检测。采用实时荧光定量逆转录聚合酶链反应（FQ-RT-PCR）分析ACE mRNA表达。我们进行了逻辑回归分析，以评估ACE I/D基因型、ACE活性及ACE mRNA表达水平与高血压的相关性。
结果
多因素逻辑回归分析结果显示，ACE基因型的加性模型（ID、DD与II相比）与高血压相关，校正后的比值比（OR）为1.43（95%可信区间：1.04-1.97），ACE ID基因型校正后的OR为1.72（95%可信区间：1.01-2.92），DD基因型校正后的OR为1.94（95%可信区间：1.01-3.73）。此外，我们的数据还表明，血浆ACE活性（校正后的OR为1.13（95%可信区间：1.08-1.18））与高血压显著相关。然而，病例组和对照组的血浆ACE mRNA表达无差异。
结论
ACE I/D多态性和ACE活性对高血压有显著影响，而在该中国人群中，循环ACE mRNA表达不是与高血压相关的重要因素。通过FQ-RT-PCR检测循环ACE mRNA表达可能是早期筛查和监测EH的一种有用方法。
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