Department of Biochemistry and Molecular Biology, Wuhan University School of Medicine, Wuhan, Hubei 430071, China.
Dis Markers. 2013;35(2):97-103. doi: 10.1155/2013/816189. Epub 2013 Jul 28.
Paraoxonase-1 (PON1), a high-density-lipoprotein- (HDL-) associated enzyme, has the potential to protect against atherogenesis. We examine the relationships between plasma PON1 activity and the progression of atherosclerosis as well as coronary artery disease (CAD). Fasting blood samples were collected from female apolipoprotein E-deficient (apoE(-/-)) mice and 149 patients undergoing coronary angiography for the biochemical parameters measurement. The severity of CAD was defined using angiographic Gensini score (GSS). Compared to 3-month-old apoE(-/-) mice, aged mice had significantly lower PON1 activity, which is negatively correlated with the size of atherosclerotic lesion and plasma interleukin-6 (IL-6) and tumor necrosis factor α (TNF- α ) levels. In study patients, PON1 activity was correlated with age, sex, and HDL-cholesterol, apolipoprotein AI, and high-sensitivity C-reactive protein (hs-CRP) levels and was significantly lower in CAD group than that in non-CAD control group. Interestingly, PON1 activity in severe CAD group (GSS > 40) was further significantly reduced compared to those in mild and moderate subgroups (GSS ≤ 40) (P < 0.01). There is a significant correlation between PON1 activity and the severity of CAD as assessed by GSS (r = -0.393, P < 0.001). PON1 activity may be a potential biomarker for the severity of CAD.
对氧磷酶 1（PON1）是一种高密度脂蛋白（HDL）相关的酶，具有防止动脉粥样硬化形成的潜力。我们研究了血浆 PON1 活性与动脉粥样硬化进展以及冠心病（CAD）之间的关系。从进行冠状动脉造影的女性载脂蛋白 E 缺陷（apoE（-/-））小鼠和 149 例患者中采集空腹血样，用于生化参数测量。CAD 的严重程度采用血管造影 Gensini 评分（GSS）定义。与 3 月龄 apoE（-/-）小鼠相比，老龄小鼠的 PON1 活性显著降低，且与动脉粥样硬化病变的大小以及血浆白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）水平呈负相关。在研究患者中，PON1 活性与年龄、性别以及 HDL-胆固醇、载脂蛋白 AI 和高敏 C 反应蛋白（hs-CRP）水平相关，且在 CAD 组中显著低于非 CAD 对照组。有趣的是，与轻度和中度亚组（GSS≤40）相比，严重 CAD 组（GSS>40）的 PON1 活性进一步显著降低（P<0.01）。PON1 活性与 GSS 评估的 CAD 严重程度之间存在显著相关性（r=-0.393，P<0.001）。PON1 活性可能是 CAD 严重程度的潜在生物标志物。
