Aryaei Ashkan, Jayatissa Ahalapitiya H, Jayasuriya Ambalangodage C
Department of Mechanical Engineering, University of Toledo, Toledo, Ohio, 43606.
J Biomed Mater Res A. 2014 Sep;102(9):3282-90. doi: 10.1002/jbm.a.34993. Epub 2013 Nov 1.
Understanding the effect of graphene substrate on graphene-cell interaction is important for considering graphene as a potential candidate for biomedical applications. In this article, biocompatibility of few layers of graphene film transferred to different substrates was evaluated using osteoblasts. The substrates were oxidized silicon wafer (SiO2/Si stack), soda lime glass, and stainless steel. Chemical vapor deposition method was employed to synthesize graphene on copper substrate using methane and hydrogen as precursors. The quality and the thickness of graphene films on different substrates were estimated by Raman spectra, whereas the thickness of graphene film was confirmed by reflectance and transmittance spectroscopy. The study was also focused on cell attachment and morphology at two time points. The results show that graphene does not have any toxic effect on osteoblasts. The cell adhesion improves with graphene coated substrate than the substrate alone. It seems that graphene substrate properties play a dominant role in cell adhesion. The result of this study suggests that a layer of graphene on bone implants will be beneficial for osteoblast attachment and proliferation.
了解石墨烯基底对石墨烯与细胞相互作用的影响对于将石墨烯视为生物医学应用的潜在候选材料至关重要。在本文中，使用成骨细胞评估了转移到不同基底上的几层石墨烯薄膜的生物相容性。基底为氧化硅晶片（SiO2/Si叠层）、钠钙玻璃和不锈钢。采用化学气相沉积法，以甲烷和氢气为前驱体在铜基底上合成石墨烯。通过拉曼光谱估计不同基底上石墨烯薄膜的质量和厚度，而通过反射率和透射率光谱确认石墨烯薄膜的厚度。该研究还聚焦于两个时间点的细胞附着和形态。结果表明，石墨烯对成骨细胞没有任何毒性作用。与单独的基底相比，涂覆有石墨烯的基底上的细胞粘附有所改善。似乎石墨烯基底特性在细胞粘附中起主导作用。本研究结果表明，骨植入物上的一层石墨烯将有利于成骨细胞的附着和增殖。
