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克隆鼠伤寒沙门氏菌表面抗原（O抗原）生物合成酶编码区域的部分片段。

Cloning part of the region encoding biosynthetic enzymes for surface antigen (O-antigen) of Salmonella typhimurium.

作者信息

Brahmbhatt H N, Quigley N B, Reeves P R

出版信息

Mol Gen Genet. 1986 Apr;203(1):172-6. doi: 10.1007/BF00330399.

DOI:10.1007/BF00330399
PMID:2423848
Abstract

The rfb gene cluster of Salmonella typhimurium encodes the enzymes required for the biosynthesis of the O-Antigen. A part of it has been cloned in plasmid vectors pBR322 and pUC9 using an adjacent, previously cloned, part of the his operon (Barnes 1981) as a molecular probe for the first clone. A detailed restriction enzyme map of 7.57 kb of rfb DNA is presented and the approximate locations of two of the genes, rfbK and rfbM have been defined.

摘要

鼠伤寒沙门氏菌的rfb基因簇编码O抗原生物合成所需的酶。利用相邻的、先前已克隆的组氨酸操纵子的一部分（Barnes，1981年）作为第一个克隆的分子探针，已将其一部分克隆到质粒载体pBR322和pUC9中。给出了7.57 kb的rfb DNA的详细限制性酶切图谱，并确定了两个基因rfbK和rfbM的大致位置。

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Cloning part of the region encoding biosynthetic enzymes for surface antigen (O-antigen) of Salmonella typhimurium.克隆鼠伤寒沙门氏菌表面抗原（O抗原）生物合成酶编码区域的部分片段。
Mol Gen Genet. 1986 Apr;203(1):172-6. doi: 10.1007/BF00330399.
2
Complete physical map of the rfb gene cluster encoding biosynthetic enzymes for the O antigen of Salmonella typhimurium LT2.编码鼠伤寒沙门氏菌LT2 O抗原生物合成酶的rfb基因簇的完整物理图谱。
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引用本文的文献

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2
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J Bacteriol. 1995 Jul;177(14):4084-8. doi: 10.1128/jb.177.14.4084-4088.1995.
3
Linkage map of Salmonella typhimurium, edition VII.

本文引用的文献

1
Fine structure mapping by complete transduction between histidine-requiring Salmonella mutants.通过在组氨酸需求型沙门氏菌突变体之间进行完全转导进行精细结构作图。
J Gen Microbiol. 1960 Apr;22:323-53. doi: 10.1099/00221287-22-2-323.
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DNA sequence of the Escherichia coli gene, gnd, for 6-phosphogluconate dehydrogenase.大肠杆菌6-磷酸葡萄糖酸脱氢酶基因（gnd）的DNA序列。
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Use of M13mp phages to study gene regulation, structure and function: cloning and recombinational analysis of genes of the Salmonella typhimurium histidine operon.
鼠伤寒沙门氏菌连锁图谱，第七版。
Microbiol Rev. 1988 Dec;52(4):485-532. doi: 10.1128/mr.52.4.485-532.1988.
4
Identification and sequence of the gene for abequose synthase, which confers antigenic specificity on group B salmonellae: homology with galactose epimerase.赋予B组沙门氏菌抗原特异性的阿比可糖合酶基因的鉴定与序列：与半乳糖差向异构酶的同源性
J Bacteriol. 1989 Oct;171(10):5687-93. doi: 10.1128/jb.171.10.5687-5693.1989.
5
Cloning and nucleotide sequence of the Salmonella typhimurium LT2 gnd gene and its homology with the corresponding sequence of Escherichia coli K12.鼠伤寒沙门氏菌LT2 gnd基因的克隆、核苷酸序列及其与大肠杆菌K12相应序列的同源性
Mol Gen Genet. 1989 May;217(1):182-4. doi: 10.1007/BF00330960.
6
Complete physical map of the rfb gene cluster encoding biosynthetic enzymes for the O antigen of Salmonella typhimurium LT2.编码鼠伤寒沙门氏菌LT2 O抗原生物合成酶的rfb基因簇的完整物理图谱。
J Bacteriol. 1988 Jan;170(1):98-102. doi: 10.1128/jb.170.1.98-102.1988.
7
O-antigen variation in Salmonella spp.: rfb gene clusters of three strains.沙门氏菌属中的O抗原变异：三株菌株的rfb基因簇
J Bacteriol. 1988 Jan;170(1):103-7. doi: 10.1128/jb.170.1.103-107.1988.
8
Genetic analysis of the O7-polysaccharide biosynthesis region from the Escherichia coli O7:K1 strain VW187.大肠杆菌O7:K1菌株VW187的O7-多糖生物合成区域的遗传分析
J Bacteriol. 1990 Jul;172(7):3590-9. doi: 10.1128/jb.172.7.3590-3599.1990.
利用M13mp噬菌体研究基因调控、结构与功能：鼠伤寒沙门氏菌组氨酸操纵子基因的克隆与重组分析
Gene. 1983 Dec;26(2-3):147-58. doi: 10.1016/0378-1119(83)90184-1.
4
Linkage map of Salmonella typhimurium, Edition VI.鼠伤寒沙门氏菌连锁图谱，第六版。
Microbiol Rev. 1983 Sep;47(3):410-53. doi: 10.1128/mr.47.3.410-453.1983.
5
Gene organization in the distal part of the Salmonella typhimurium histidine operon and determination and sequence of the operon transcription terminator.鼠伤寒沙门氏菌组氨酸操纵子远端部分的基因组织以及操纵子转录终止子的确定和序列
Mol Gen Genet. 1983;191(3):413-20. doi: 10.1007/BF00425756.
6
pEMBL: a new family of single stranded plasmids.pEMBL：一类新型单链质粒。
Nucleic Acids Res. 1983 Mar 25;11(6):1645-55. doi: 10.1093/nar/11.6.1645.
7
Cloning and expression of the distal portion of the histidine operon of Escherichia coli K-12.大肠杆菌K-12组氨酸操纵子远端部分的克隆与表达。
J Bacteriol. 1982 Aug;151(2):692-700. doi: 10.1128/jb.151.2.692-700.1982.
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Cloning and restriction map of the first part of the histidine operon of Salmonella typhimurium.鼠伤寒沙门氏菌组氨酸操纵子第一部分的克隆与限制酶图谱
J Bacteriol. 1981 Jul;147(1):124-34. doi: 10.1128/jb.147.1.124-134.1981.
9
A rapid and efficient procedure for the purification of DNA from agarose gels.一种从琼脂糖凝胶中快速高效纯化DNA的方法。
Anal Biochem. 1980 Aug;106(2):492-6. doi: 10.1016/0003-2697(80)90553-9.
10
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Gene. 1980 Feb;8(3):239-53. doi: 10.1016/0378-1119(80)90002-5.