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利用针对核衣壳蛋白的单克隆抗体对狂犬病病毒分离株进行抗原变异的证明。

Demonstration of antigenic variation among rabies virus isolates by using monoclonal antibodies to nucleocapsid proteins.

作者信息

Smith J S, Reid-Sanden F L, Roumillat L F, Trimarchi C, Clark K, Baer G M, Winkler W G

出版信息

J Clin Microbiol. 1986 Oct;24(4):573-80. doi: 10.1128/jcm.24.4.573-580.1986.

Abstract

Rabies virus isolates from terrestrial animals in six areas of the United States were examined with a panel of monoclonal antibodies to nucleocapsid proteins. Characteristic differences in immunofluorescence reactions permitted the formation of four antigenically distinct reaction groups from the 231 isolates tested. The geographic distribution of these groups corresponded well with separate rabies enzootic areas recognized by surveillance of sylvatic rabies in the United States. Distinctive reaction patterns were also identified for viral proteins from four infected bat species, and identical patterns were found in eight isolated cases of rabies in terrestrial animals. These findings suggest that monoclonal antibodies can be used to study the prevalence, distribution, and transmission of rabies among wildlife species.

摘要

利用一组针对核衣壳蛋白的单克隆抗体,对来自美国六个地区陆生动物的狂犬病病毒分离株进行了检测。免疫荧光反应的特征差异使得在所检测的231个分离株中形成了四个抗原性不同的反应组。这些组的地理分布与美国通过监测野生动物狂犬病所确认的不同狂犬病疫区非常吻合。还确定了四种受感染蝙蝠物种病毒蛋白的独特反应模式,并且在八例陆生动物狂犬病孤立病例中发现了相同的模式。这些发现表明,单克隆抗体可用于研究野生动物物种中狂犬病的流行、分布和传播情况。

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本文引用的文献

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Rabies in bats in Florida.佛罗里达州蝙蝠身上的狂犬病
Am J Public Health Nations Health. 1954 Feb;44(2):182-5. doi: 10.2105/ajph.44.2.182.
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A case of equine rabies.一例马狂犬病病例。
N Engl J Med. 1984 Feb 23;310(8):525-6. doi: 10.1056/NEJM198402233100812.
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Rabies in translocated raccoons.迁移浣熊中的狂犬病
Am J Public Health. 1979 Jun;69(6):601-2. doi: 10.2105/ajph.69.6.601.

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