Kim Beob Soo, Kim Eun Ji, Choi Ji Suk, Jeong Ji Hoon, Jo Chris Hyunchul, Cho Yong Woo
Department of Chemical Engineering, Hanyang University, Hanyangdaehak-ro 55, Ansan, Kyeonggi-do 426-791, Republic of Korea; Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seoul, 136-791, Republic of Korea.
J Biomed Mater Res A. 2014 Nov;102(11):4044-54. doi: 10.1002/jbm.a.35057. Epub 2014 Jan 17.
The natural tendon-to-bone region has a gradient in structure and composition, which is translated into a spatial variation of chemical, physical, and biological properties. This unique transitional tissue between bone and tendon is not normally recreated during natural bone-to-tendon healing. In this study, we have developed a human collagen-based multilayer scaffold mimicking the tendon-to-bone region. The scaffold consists of four different layers with the following composition gradient: (a) a tendon layer composed of collagen; (b) an uncalcified fibrocartilage layer composed of collagen and chondroitin sulfate; (c) a calcified fibrocartilage layer composed of collagen and less apatite; (d) a bone layer composed of collagen and apatite. The chemical, physical, and mechanical properties of the scaffold were characterized by a scanning electron microscope, porosimeter, universal tensile machine, Fourier transform infrared spectrometer, energy dispersive X-ray analysis apparatus, and thermogravimetric analysis apparatus. The multilayer scaffold provided a gradual transition of the physical, chemical, and mechanical environment and supported the adhesion and proliferation of human fibroblasts, chondrocytes, and osteoblasts toward each corresponding matrix. Overall, our results suggest the feasibility of a human collagen-based multilayer scaffold for regeneration of hard-to-soft interface tissues.
天然的肌腱-骨区域在结构和组成上存在梯度变化，这转化为化学、物理和生物学特性的空间变化。这种在骨与肌腱之间独特的过渡组织在自然的骨-肌腱愈合过程中通常不会重现。在本研究中，我们开发了一种模仿肌腱-骨区域的基于人胶原蛋白的多层支架。该支架由四个不同的层组成，具有以下组成梯度：(a) 由胶原蛋白组成的肌腱层；(b) 由胶原蛋白和硫酸软骨素组成的未钙化纤维软骨层；(c) 由胶原蛋白和较少磷灰石组成的钙化纤维软骨层；(d) 由胶原蛋白和磷灰石组成的骨层。通过扫描电子显微镜、孔隙率测定仪、万能拉伸机、傅里叶变换红外光谱仪、能量色散X射线分析仪和热重分析仪对支架的化学、物理和力学性能进行了表征。多层支架提供了物理、化学和力学环境的逐渐过渡，并支持人成纤维细胞、软骨细胞和成骨细胞向各自相应基质的黏附和增殖。总体而言，我们的结果表明基于人胶原蛋白的多层支架用于再生硬-软界面组织的可行性。
