Aydin Suna, Kuloglu Tuncay, Aydin Suleyman, Eren Mehmet Nesimi, Celik Ahmet, Yilmaz Musa, Kalayci Mehmet, Sahin İbrahim, Gungor Orhan, Gurel Ali, Ogeturk Murat, Dabak Ozlem
Department of Cardiovascular Surgery, Elazig Research and Education Hospital, Elazig 23100, Turkey; Firat University, School of Medicine, Department of Anatomy, Elazig 23119, Turkey.
Firat University, School of Medicine, Department of Histology and Embryology, Elazig 23119, Turkey.
Peptides. 2014 Feb;52:68-73. doi: 10.1016/j.peptides.2013.11.024. Epub 2013 Dec 15.
Irisin converts white adipose tissue (WAT) into brown adipose tissue (BAT), as regulated by energy expenditure. The relationship between irisin concentrations after exercise in rats compared humans after exercise remains controversial. We therefore: (1) measured irisin expression in cardiac and skeletal muscle, liver, kidney, peripheral nerve sheath and skin tissues, as also serum irisin level in 10 week-old rats without exercise, and (2) measured tissue supernatant irisin levels in cardiac and skeletal muscle, and in response to exercise in young and old rats to establishing which tissues produced most irisin. Young (12 months) and old rats (24 months) with or without 10min exercise (water floating) and healthy 10 week-old Sprague-Dawley rats without exercise were used. Irisin was absent from sections of skeletal muscle of unexercised rats, the only part being stained being the perimysium. In contrast, cardiac muscle tissue, peripheral myelin sheath, liver, kidneys, and skin dermis and hypodermis were strongly immunoreactivity. No irisin was seen in skeletal muscle of unexercised young and old rats, but a slight amount was detected after exercise. Strong immunoreactivity occurred in cardiac muscle of young and old rats with or without exercise, notably in pericardial connective tissue. Serum irisin increased after exercise, being higher in younger than older rats. Irisin in tissue supernatants (cardiac and skeletal muscle) was high with or without exercise. High supernatant irisin could come from connective tissues around skeletal muscle, especially nerve sheaths located within it. Skeletal muscle is probably not a main irisin source.
鸢尾素可将白色脂肪组织（WAT）转化为棕色脂肪组织（BAT），这一过程受能量消耗调节。运动后大鼠体内鸢尾素浓度与人类运动后鸢尾素浓度之间的关系仍存在争议。因此，我们：（1）测量了10周龄未运动大鼠的心脏、骨骼肌、肝脏、肾脏、外周神经鞘和皮肤组织中的鸢尾素表达以及血清鸢尾素水平，（2）测量了年轻和老年大鼠心脏和骨骼肌组织上清液中的鸢尾素水平，并观察运动对其的影响，以确定哪些组织产生的鸢尾素最多。使用了12个月龄（年轻）和24个月龄（老年）的大鼠，分别进行10分钟的运动（漂浮于水中）或不运动，以及10周龄健康未运动的斯普拉格-道利大鼠。未运动大鼠的骨骼肌切片中没有鸢尾素，唯一染色的部分是肌束膜。相比之下，心肌组织、外周髓鞘、肝脏、肾脏以及皮肤真皮和皮下组织有强烈的免疫反应性。未运动的年轻和老年大鼠的骨骼肌中未见鸢尾素，但运动后检测到少量鸢尾素。无论运动与否，年轻和老年大鼠的心肌均有强烈的免疫反应性，尤其是在心包结缔组织中。运动后血清鸢尾素增加，年轻大鼠高于老年大鼠。无论运动与否，组织上清液（心脏和骨骼肌）中的鸢尾素都很高。高组织上清鸢尾素可能来自骨骼肌周围的结缔组织，尤其是其中的神经鞘。骨骼肌可能不是鸢尾素的主要来源。
