Gür F M, Timurkaan S, Yalcin M H, Girgin A, Gençer Tarakçı B
a Department of Histology and Embryology , School of Medicine, Ömer Halisdemir University , Nigde.
b Department of Histology and Embryology, Faculty of Veterinary Medicine , Firat University , Elazig , Turkey.
Biotech Histochem. 2017;92(4):245-251. doi: 10.1080/10520295.2017.1303194. Epub 2017 Apr 14.
Irisin was first identified in skeletal muscle cells. It is an exercise protein that is secreted into the circulation; it causes conversion of white adipose tissue to brown adipose tissue. We investigated irisin immunoreactivity in mole rat (Spalax leucodon) tissues. We examined cerebellum, pituitary, heart, liver, pancreas, spleen, uterus, kidney and striated muscle in female adult mole rats. Tissues were processed, embedded in paraffin, sectioned at 5 μm and stained immunohistochemically for irisin. Irisin immunostaining was detected in the cytoplasm of stained cells; the cytoplasm of Purkinje cells was unstained. We found that irisin may be synthesized in many tissues. The function of locally synthesized irisin currently is unknown.
鸢尾素最初是在骨骼肌细胞中被发现的。它是一种分泌到循环系统中的运动蛋白；它能使白色脂肪组织转化为棕色脂肪组织。我们研究了盲鼹鼠（斯帕拉克斯白齿鼹）组织中的鸢尾素免疫反应性。我们检查了成年雌性盲鼹鼠的小脑、垂体、心脏、肝脏、胰腺、脾脏、子宫、肾脏和横纹肌。组织经过处理，嵌入石蜡，切成5微米厚的切片，并进行鸢尾素免疫组织化学染色。在染色细胞的细胞质中检测到鸢尾素免疫染色；浦肯野细胞的细胞质未染色。我们发现鸢尾素可能在许多组织中合成。目前尚不清楚局部合成的鸢尾素的功能。
