Optical Imaging Laboratory, Department of Biomedical Engineering and Department of Radiology, Washington University in St. Louis, St. Louis, MO 63130.
Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):21-6. doi: 10.1073/pnas.1311868111. Epub 2013 Dec 23.
The increasing use of mouse models for human brain disease studies presents an emerging need for a new functional imaging modality. Using optical excitation and acoustic detection, we developed a functional connectivity photoacoustic tomography system, which allows noninvasive imaging of resting-state functional connectivity in the mouse brain, with a large field of view and a high spatial resolution. Bilateral correlations were observed in eight functional regions, including the olfactory bulb, limbic, parietal, somatosensory, retrosplenial, visual, motor, and temporal regions, as well as in several subregions. The borders and locations of these regions agreed well with the Paxinos mouse brain atlas. By subjecting the mouse to alternating hyperoxic and hypoxic conditions, strong and weak functional connectivities were observed, respectively. In addition to connectivity images, vascular images were simultaneously acquired. These studies show that functional connectivity photoacoustic tomography is a promising, noninvasive technique for functional imaging of the mouse brain.
越来越多的人使用小鼠模型来研究人类脑部疾病，这就对一种新的功能成像模式提出了新的需求。我们利用光激发和声学检测技术，开发了一种功能连接性光声断层成像系统，该系统允许对小鼠大脑的静息状态功能连接进行非侵入性成像，具有大的视野和高的空间分辨率。在包括嗅球、边缘系统、顶叶、感觉运动、后穹窿、视觉、运动和颞叶在内的八个功能区域以及几个亚区域中观察到了双侧相关性。这些区域的边界和位置与 Paxinos 小鼠脑图谱吻合良好。通过使小鼠交替处于高氧和低氧条件下，分别观察到了强和弱的功能连接。除了连接图像外，还同时获得了血管图像。这些研究表明，功能连接性光声断层成像术是一种很有前途的、用于小鼠大脑功能成像的非侵入性技术。
