1] Department of Chemistry, University of Oxford, Oxford, UK. [2] Center for DNA Nanotechnology, Department of Chemistry and iNANO, Aarhus University, Aarhus, Denmark. [3].
1] Department of Chemistry, University of Oxford, Oxford, UK. [2].
Nat Biotechnol. 2014 Feb;32(2):179-81. doi: 10.1038/nbt.2799. Epub 2014 Jan 19.
We demonstrate single-molecule, site-specific detection of protein phosphorylation with protein nanopore technology. A model protein, thioredoxin, was phosphorylated at two adjacent sites. Analysis of the ionic current amplitude and noise, as the protein unfolds and moves through an α-hemolysin pore, enables the distinction between unphosphorylated, monophosphorylated and diphosphorylated variants. Our results provide a step toward nanopore proteomics.
我们展示了使用蛋白质纳米孔技术对蛋白质磷酸化进行单分子、特异性检测。选择一种模型蛋白——硫氧还蛋白，在两个相邻的位点进行磷酸化。分析蛋白质在穿过α-溶血素孔时展开和移动过程中离子电流幅度和噪声的变化，可以区分未磷酸化、单磷酸化和双磷酸化的变体。我们的研究结果为蛋白质纳米孔组学研究迈出了一步。
