酵母中的正交 DNA 复制系统。
An orthogonal DNA replication system in yeast.
机构信息
Department of Biomedical Engineering, University of California-Irvine, Irvine, California, USA.
1] Department of Biomedical Engineering, University of California-Irvine, Irvine, California, USA. [2] Department of Chemistry, University of California-Irvine, Irvine, California, USA. [3] Center for Complex Biological Systems, University of California-Irvine, Irvine, California, USA.
出版信息
Nat Chem Biol. 2014 Mar;10(3):175-7. doi: 10.1038/nchembio.1439. Epub 2014 Feb 2.
An extranuclear replication system, consisting of an orthogonal DNA plasmid-DNA polymerase pair, was developed in Saccharomyces cerevisiae. Engineered error-prone DNA polymerases showed complete mutational targeting in vivo: per-base mutation rates on the plasmid were increased substantially and remained stable with no increase in genomic rates. Orthogonal replication serves as a platform for in vivo continuous evolution and as a system whose replicative properties can be manipulated independently of the host's.
在酿酒酵母中开发了一种由正交 DNA 质粒-DNA 聚合酶对组成的核外复制系统。经过工程改造的易错 DNA 聚合酶在体内表现出完全的突变靶向性：质粒上的每个碱基的突变率显著增加，而基因组的突变率没有增加，保持稳定。正交复制是体内连续进化的平台，也是一种可以独立于宿主操纵其复制特性的系统。
Zotero 插件