School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, Zhejiang, China.
PLoS One. 2014 Feb 5;9(2):e87996. doi: 10.1371/journal.pone.0087996. eCollection 2014.
To evaluate the repeatability, reproducibility, and agreement of thickness profile measurements of eight intra-retinal layers determined by an automated algorithm applied to optical coherence tomography (OCT) images from two different instruments.
Twenty normal subjects (12 males, 8 females; 24 to 32 years old) were enrolled. Imaging was performed with a custom built ultra-high resolution OCT instrument (UHR-OCT, ∼3 µm resolution) and a commercial RTVue100 OCT (∼5 µm resolution) instrument. An automated algorithm was developed to segment the macular retina into eight layers and quantitate the thickness of each layer. The right eye of each subject was imaged two times by the first examiner using each instrument to assess intra-observer repeatability and once by the second examiner to assess inter-observer reproducibility. The intraclass correlation coefficient (ICC) and coefficients of repeatability and reproducibility (COR) were analyzed to evaluate the reliability.
The ICCs for the intra-observer repeatability and inter-observer reproducibility of both SD-OCT instruments were greater than 0.945 for the total retina and all intra-retinal layers, except the photoreceptor inner segments, which ranged from 0.051 to 0.643, and the outer segments, which ranged from 0.709 to 0.959. The CORs were less than 6.73% for the total retina and all intra-retinal layers. The total retinal thickness measured by the UHR-OCT was significantly thinner than that measured by the RTVue100. However, the ICC for agreement of the thickness profiles between UHR-OCT and RTVue OCT were greater than 0.80 except for the inner segment and outer segment layers.
Thickness measurements of the intra-retinal layers determined by the automated algorithm are reliable when applied to images acquired by the UHR-OCT and RTVue100 instruments.
评估应用于两种不同仪器的光学相干断层扫描（OCT）图像的自动算法测量的 8 个视网膜内厚度剖面的重复性、可再现性和一致性。
共纳入 20 名正常受试者（12 名男性，8 名女性；24 至 32 岁）。使用定制的超高分辨率 OCT 仪器（UHR-OCT，约 3μm 分辨率）和商用 RTVue100 OCT 仪器（约 5μm 分辨率）进行成像。开发了一种自动算法来将黄斑视网膜分割成 8 层，并定量测量每一层的厚度。每位受试者的右眼由第一检查者使用两种仪器各检查两次，以评估观察者内重复性，由第二检查者检查一次，以评估观察者间可再现性。采用组内相关系数（ICC）和重复性和再现性系数（COR）来分析评估可靠性。
两种 SD-OCT 仪器的观察者内重复性和观察者间可再现性的 ICC 均大于 0.945，适用于全视网膜和所有视网膜内各层，除了内节和外节，范围分别为 0.051 至 0.643 和 0.709 至 0.959。全视网膜和所有视网膜内各层的 COR 均小于 6.73%。UHR-OCT 测量的总视网膜厚度明显比 RTVue100 薄。然而，UHR-OCT 和 RTVue OCT 之间厚度剖面的一致性的 ICC 大于 0.80，除了内节和外节层。
应用于 UHR-OCT 和 RTVue100 仪器获取的图像时，自动算法确定的视网膜内各层的厚度测量具有可靠性。
