Department of Rehabilitation Medicine, College of Korean Medicine, Daegu Haany University, 1 Hannydaero, Gyeongsan, Gyeongsangbuk-Do 712-715, Republic of Korea.
The Medical Research Center for Globalization of Herbal Medicine, College of Korean Medicine, Daegu Haany University, 1 Hannydaero, Gyeongsan, Gyeongsangbuk-Do 712-715, Republic of Korea ; Department of Histology and Anatomy, College of Korean Medicine, Daegu Haany University, 1 Hannydaero, Gyeongsan, Gyeongsangbuk-Do 712-715, Republic of Korea.
Evid Based Complement Alternat Med. 2014;2014:954508. doi: 10.1155/2014/954508. Epub 2014 Jan 6.
The object of this study is to observe the effects of platycodin D, a saponin purified from Platycodi Radix, on mice collagen-induced arthritis (CIA). A daily dose of 200, 100, and 50 mg/kg platycodin D was administered orally to male DBA/1J mice for 40 days after initial collagen immunization. To ascertain the effects administering the collagen booster, CIA-related features (including body weight, poly-arthritis, knee and paw thickness, and paw weight increase) was measured from histopathological changes in the spleen, left popliteal lymph node, third digit, and the knee joint regions. CIA-related bone and cartilage damage improved significantly in the platycodin D-administered CIA mice. Additionally, myeloperoxidase (MPO) levels in the paw were reduced in platycodin D-treated CIA mice compared to CIA control groups. The level of malondialdehyde (MDA), an indicator of oxidative stress, decreased in a dose-dependent manner in the platycodin D group. Finally, the production of IL-6 and TNF- α , involved in rheumatoid arthritis pathogenesis, was suppressed by treatment with platycodin D. Taken together, these results suggest that platycodin D is a promising new effective antirheumatoid arthritis agent, exerting anti-inflammatory, antioxidative and immunomodulatory effects in CIA mice.
本研究旨在观察桔梗皂苷 D（从桔梗中提取的一种皂苷）对胶原诱导性关节炎（CIA）小鼠的作用。雄性 DBA/1J 小鼠在初次胶原免疫后 40 天内，每天口服给予 200、100 和 50mg/kg 的桔梗皂苷 D。为了确定给予胶原增强剂的效果，从脾脏、左腘淋巴结、第三指和膝关节区域的组织病理学变化来测量与 CIA 相关的特征（包括体重、多发性关节炎、膝关节和爪子厚度以及爪子重量增加）。在接受桔梗皂苷 D 治疗的 CIA 小鼠中，与 CIA 对照组相比，CIA 相关的骨和软骨损伤显著改善。此外，与 CIA 对照组相比，在接受桔梗皂苷 D 治疗的 CIA 小鼠中，爪子中的髓过氧化物酶（MPO）水平降低。丙二醛（MDA）水平呈剂量依赖性降低，MDA 是氧化应激的指标。最后，参与类风湿性关节炎发病机制的 IL-6 和 TNF-α 的产生被桔梗皂苷 D 的治疗所抑制。综上所述，这些结果表明，桔梗皂苷 D 是一种有前途的新型有效的抗类风湿关节炎药物，在 CIA 小鼠中具有抗炎、抗氧化和免疫调节作用。
