Demian Emese, Jaksa-Czotter Nikoletta, Varallyay Eva
Genomics Research Group, Department of Plant Pathology, Institute of Plant Protection, Hungarian University of Agriculture and Life Sciences, 2100 Godollo, Hungary.
Plants (Basel). 2022 Jul 12;11(14):1830. doi: 10.3390/plants11141830.
Grapevine Pinot gris virus (GPGV) was described in Italy using a metagenomic approach: next-generation sequencing of the virus-derived small RNAs. Since that time, it has been reported all over the world. The presence of GPGV is associated with grapevine disease, but most of the time, the disease is asymptomatic. Although the host range of this virus has not been investigated, it has been found in the non- hosts, and . We investigated the presence of GPGV in grapevine and other plant species growing as weeds in the vineyard. Using RT-PCR, we identified GPGV in seven non- hosts: , , , , , , and . In the case of and , this finding was supported by Northern blot detection of the virus. GPGV strains in non- hosts belong to the asymptomatic clade, and are clustered according to their original geographic locations. The presence of GPGV in species other than grapevine shows that besides well-known vector and propagating material-based infections, other possible entry sites for the virus can exist, which have to be taken into consideration when developing reliable regulation strategies.
葡萄灰比诺病毒（GPGV）是在意大利通过宏基因组学方法发现的：对病毒衍生的小RNA进行下一代测序。自那时起，它已在世界各地被报道。GPGV的存在与葡萄疾病有关，但大多数情况下，这种疾病是无症状的。尽管尚未对该病毒的宿主范围进行研究，但已在非宿主植物中发现了它。我们调查了葡萄园里作为杂草生长的葡萄及其他植物物种中GPGV的存在情况。通过逆转录聚合酶链反应（RT-PCR），我们在七种非宿主植物中鉴定出了GPGV，分别是[此处原文缺失七种非宿主植物的具体名称]。就[此处原文缺失两种植物的具体名称]而言，病毒的Northern印迹检测结果支持了这一发现。非宿主植物中的GPGV毒株属于无症状进化枝，并根据其原始地理位置聚类。葡萄以外的物种中存在GPGV表明，除了众所周知的通过媒介和繁殖材料传播的感染途径外，病毒可能还有其他进入途径，在制定可靠的监管策略时必须考虑到这些途径。
