Kim Kyoung-Ho, Ten Leonid N, Liu Qing-Mei, Im Wan-Taek, Lee Sung-Taik
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
J Microbiol. 2008 Aug;46(4):390-5. doi: 10.1007/s12275-008-0019-0. Epub 2008 Aug 31.
A Gram-negative, motile, rod-shaped, non-spore-forming bacterial strain, designated as Ko03(T), was isolated from microbial granules, and was characterized, using a polyphasic approach, in order to determine its taxonomic position. The isolate was positive for catalase and oxidase, but negative for gelatinase and beta-galactosidase. Phylogenetic analyses using the 16S rRNA gene sequence showed that the strain formed a monophyletic branch towards the periphery of the evolutionary radiation occupied by the genus Comamonas, its closest neighbors being Comamonas koreensis KCTC 12005(T) (95.9% sequence similarity), Comamonas nitrativorans DSM 13191(T) (95.7%), and Comamonas odontotermitis LMG 23579(T) (95.7%). Strain Ko03(T) had a genomic DNA G+C content of 68.4 mol% and the predominant respiratory quinone was Q-8. The major fatty acids were C(16:1) omega7c (44.7%), C(16:0) (28.1%), C(18:1) (16.1%), and C(10:0) 3-OH (3.5%). These chemo-taxonomic results supported the affiliation of strain Ko03(T) to the genus Comamonas. However, low 16S rRNA gene sequence similarity values and distinguishing phenotypic characteristics allowed genotypic and phenotypic differentiation of strain Ko03(T) from recognized Comamonas species. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Ko03(T) represents a novel species of the genus Comamonas, for which the name Comamonas granuli sp. nov. is proposed. The type strain is Ko03(T) (=KCTC 12199(T)=NBRC 101663(T)).
从微生物颗粒中分离出一株革兰氏阴性、具运动性、杆状、不产芽孢的细菌菌株，命名为Ko03(T)，并采用多相分类法对其进行表征，以确定其分类地位。该分离株过氧化氢酶和氧化酶呈阳性，但明胶酶和β-半乳糖苷酶呈阴性。使用16S rRNA基因序列进行的系统发育分析表明，该菌株在丛毛单胞菌属占据的进化辐射边缘形成一个单系分支，其最接近的亲缘种为韩国丛毛单胞菌KCTC 12005(T)（序列相似性为95.9%）、硝酸丛毛单胞菌DSM 13191(T)（95.7%）和白蚁丛毛单胞菌LMG 23579(T)（95.7%）。菌株Ko03(T)的基因组DNA G+C含量为68.4 mol%，主要呼吸醌为Q-8。主要脂肪酸为C(16:1) ω7c（44.7%）、C(16:0)（28.1%）、C(18:1)（16.1%）和C(10:0) 3-OH（3.5%）。这些化学分类学结果支持菌株Ko03(T)隶属于丛毛单胞菌属。然而，低的16S rRNA基因序列相似性值和独特的表型特征使得菌株Ko03(T)与已确认的丛毛单胞菌属物种在基因型和表型上存在差异。基于其表型特性和系统发育独特性，菌株Ko03(T)代表丛毛单胞菌属的一个新物种，为此提出新名称颗粒丛毛单胞菌（Comamonas granuli sp. nov.）。模式菌株为Ko03(T)（=KCTC 12199(T)=NBRC 101663(T)）。
