Li Quan-Li, Ning Tian-Yun, Cao Ying, Zhang Wei-bo, Mei May Lei, Chu Chun Hung
College & Hospital of Stomatology, Anhui Medical University, 230032 Hefei, China.
BMC Biotechnol. 2014 Apr 27;14:32. doi: 10.1186/1472-6750-14-32.
Researchers are looking for biomimetic mineralization of ena/mel to manage dental erosion. This study evaluated biomimetic mineralization of demineralized enamel induced by a synthetic and self-assembled oligopeptide amphiphile (OPA).
The results showed that the OPA self-assembled into nano-fibres in the presence of calcium ions and in neutral acidity. The OPA was alternately immersed in calcium chloride and sodium hypophosphate solutions to evaluate its property of mineralization. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) showed nucleation and growth of amorphous calcium phosphate along the self-assembled OPA nano-fibres when it was repetitively exposed to solutions with calcium and phosphate ions. Energy dispersive spectrometry (EDS) confirmed that these nano-particles contained calcium and phosphate. Furthermore, electron diffraction pattern suggested that the nano-particles precipitated on OPA nano-fibres were comparable to amorphous calcium phosphate. Acid-etched human enamel slices were incubated at 37°C in metastable calcium phosphate solution with the OPA for biomimetic mineralization. SEM and X-ray diffraction indicated that the OPA induced the formation of hydroxyapatite crystals in organized bundles on etched enamel. TEM micrographs revealed there were 20-30 nm nano-amorphous calcium phosphate precipitates in the biomimetic mineralizing solution. The particles were found separately bound to the oligopeptide fibres. Biomimetic mineralization with or without the oligopeptide increased demineralized enamel microhardness.
A novel OPA was successfully fabricated, which fostered the biomimetic mineralization of demineralized enamel. It is one of the primary steps towards the design and construction of novel biomaterial for future clinical therapy of dental erosion.
研究人员正在寻找牙釉质/牙本质的仿生矿化方法来处理牙侵蚀问题。本研究评估了一种合成的自组装寡肽两亲分子（OPA）诱导脱矿牙釉质的仿生矿化情况。
结果表明，OPA在钙离子存在及中性酸度条件下自组装成纳米纤维。将OPA交替浸入氯化钙和次磷酸钠溶液中以评估其矿化性能。透射电子显微镜（TEM）和扫描电子显微镜（SEM）显示，当OPA重复暴露于含有钙离子和磷酸根离子的溶液中时，无定形磷酸钙会沿着自组装的OPA纳米纤维成核并生长。能量色散光谱（EDS）证实这些纳米颗粒含有钙和磷。此外，电子衍射图谱表明沉淀在OPA纳米纤维上的纳米颗粒与无定形磷酸钙相当。将酸蚀后的人牙釉质切片在37°C下于含有OPA的亚稳磷酸钙溶液中孵育以进行仿生矿化。SEM和X射线衍射表明，OPA诱导在蚀刻后的牙釉质上形成有组织的束状羟基磷灰石晶体。TEM显微照片显示，仿生矿化溶液中存在20 - 30 nm的纳米无定形磷酸钙沉淀。发现这些颗粒分别与寡肽纤维结合。有或没有寡肽的仿生矿化均提高了脱矿牙釉质的显微硬度。
成功制备了一种新型OPA，其促进了脱矿牙釉质的仿生矿化。这是迈向设计和构建用于未来牙侵蚀临床治疗的新型生物材料的主要步骤之一。
