Pinho Eva, Ferreira Isabel C F R, Barros Lillian, Carvalho Ana Maria, Soares Graça, Henriques Mariana
CEB, Centre for Biological Engineering, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal ; Centre for Textile Science and Technology (2C2T), University of Minho, Campus Azurém, 4800-058 Guimarães, Portugal.
CIMO/School of Agriculture, Polytechnic Institute of Bragança, Campus de Santa Apolónia, 172, 5301-855 Bragança, Portugal.
Biomed Res Int. 2014;2014:814590. doi: 10.1155/2014/814590. Epub 2014 Apr 8.
The present work aims to assess the antibacterial potential of phenolic extracts, recovered from plants obtained on the North East of Portugal, and of their phenolic compounds (ellagic, caffeic, and gallic acids, quercetin, kaempferol, and rutin), against bacteria commonly found on skin infections. The disk diffusion and the susceptibility assays were used to identify the most active extracts and phenolic compounds. The effect of selected phenolic compounds on animal cells was assessed by determination of cellular metabolic activity. Gallic acid had a higher activity, against gram-positive (S. epidermidis and S. aureus) and gram-negative bacteria (K. pneumoniae) at lower concentrations, than the other compounds. The caffeic acid, also, showed good antibacterial activity against the 3 bacteria used. The gallic acid was effective against the 3 bacteria without causing harm to the animal cells. Gallic and caffeic acid showed a promising applicability as antibacterial agents for the treatment of infected wounds.
本研究旨在评估从葡萄牙东北部采集的植物中提取的酚类提取物及其酚类化合物（鞣花酸、咖啡酸、没食子酸、槲皮素、山奈酚和芦丁）对皮肤感染常见细菌的抗菌潜力。采用纸片扩散法和药敏试验来鉴定最具活性的提取物和酚类化合物。通过测定细胞代谢活性来评估所选酚类化合物对动物细胞的影响。没食子酸在较低浓度下对革兰氏阳性菌（表皮葡萄球菌和金黄色葡萄球菌）和革兰氏阴性菌（肺炎克雷伯菌）的活性高于其他化合物。咖啡酸对所使用的3种细菌也表现出良好的抗菌活性。没食子酸对这3种细菌有效，且不会对动物细胞造成伤害。没食子酸和咖啡酸作为治疗感染伤口的抗菌剂显示出有前景的适用性。
