College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.
World J Microbiol Biotechnol. 2012 May;28(5):2077-86. doi: 10.1007/s11274-012-1010-9. Epub 2012 Feb 11.
Nicotine is a key harmful component of tobacco and cigarettes, and the development of low-nicotine cigarettes is of increasing importance in the market. The objectives of this study are to isolate native nicotine-degrading strains and evaluate their feasibility for nicotine reduction during the aging (or fermentation) of tobacco leaves. A novel nicotine-degrading strain was isolated and identified as Pseudomonas stutzeri ZCJ based on its 16S rDNA sequence and morphological-biochemical characteristics. In submerged cultures, P. stutzeri ZCJ could tolerate 4.5 g/L nicotine and completely degrade 1.5 g/L nicotine within 24 h at 37°C and pH 7.4. The addition of glucose (1 g/L) could improve nicotine degradation by P. stutzeri ZCJ in submerged cultures. After submerged culturing, the cell suspension of P. stutzeri ZCJ could be utilized to improve nicotine reduction in tobacco leaves during solid-state fermentation. The nicotine content of tobacco leaves decreased by as much as 32.24% after 7 days of solid-state fermentation by P. stutzeri ZCJ, suggesting the industrial application potential of the native strain to enhance nicotine degradation during the aging of tobacco leaves.
尼古丁是烟草和香烟的主要有害成分，开发低尼古丁香烟在市场上变得越来越重要。本研究的目的是分离本土尼古丁降解菌株，并评估它们在烟叶老化（或发酵）过程中降低尼古丁的可行性。根据 16S rDNA 序列和形态生化特征，从烟叶中分离并鉴定出一株新型尼古丁降解菌，命名为施氏假单胞菌 ZCJ。在液体培养中，施氏假单胞菌 ZCJ 能耐受 4.5 g/L 的尼古丁，并在 37°C 和 pH 值 7.4 下 24 h 内完全降解 1.5 g/L 的尼古丁。在液体培养中添加葡萄糖（1 g/L）可提高施氏假单胞菌 ZCJ 的尼古丁降解能力。液体培养后，可将施氏假单胞菌 ZCJ 的细胞悬浮液用于提高固态发酵过程中烟叶中尼古丁的还原。施氏假单胞菌 ZCJ 固态发酵 7 天后，烟叶中的尼古丁含量降低了 32.24%，表明该本土菌株在增强烟叶老化过程中尼古丁降解方面具有工业应用潜力。
