Smith A S T, Long C J, Pirozzi K, Najjar S, McAleer C, Vandenburgh H H, Hickman J J
NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Orlando, FL 32826, USA.
Department of Pathology and Laboratory Medicine, Brown University, Providence, RI 02906, USA.
J Biotechnol. 2014 Sep 20;185:15-8. doi: 10.1016/j.jbiotec.2014.05.029. Epub 2014 Jun 5.
This report details the development of a non-invasive in vitro assay system for investigating the functional maturation and performance of human skeletal myotubes. Data is presented demonstrating the survival and differentiation of human myotubes on microscale silicon cantilevers in a defined, serum-free system. These cultures can be stimulated electrically and the resulting contraction quantified using modified atomic force microscopy technology. This system provides a higher degree of sensitivity for investigating contractile waveforms than video-based analysis, and represents the first system capable of measuring the contractile activity of individual human muscle myotubes in a reliable, high-throughput and non-invasive manner. The development of such a technique is critical for the advancement of body-on-a-chip platforms toward application in pre-clinical drug development screens.
本报告详细介绍了一种用于研究人骨骼肌肌管功能成熟和性能的非侵入性体外分析系统的开发。文中展示的数据表明，在特定的无血清系统中，人肌管在微尺度硅悬臂上能够存活并分化。这些培养物可以进行电刺激，并使用改进的原子力显微镜技术对产生的收缩进行量化。与基于视频的分析相比，该系统在研究收缩波形方面具有更高的灵敏度，并且是首个能够以可靠、高通量和非侵入性方式测量单个人类肌肉肌管收缩活性的系统。这种技术的发展对于片上人体平台在临床前药物开发筛选中的应用推进至关重要。
