Hong Seong Su, Lee Ji Eun, Jung Yeon Woo, Park Ju-Hyoung, Lee Jung A, Jeong Wonsik, Ahn Eun-Kyung, Choi Chun Whan, Oh Joa Sub
Bio-Center, Gyeonggido Business & Science Accelerator (GBSA), Suwon 16229, Korea.
College of Pharmacy, Dankook University, Cheonan 31116, Korea.
Plants (Basel). 2021 Jan 28;10(2):257. doi: 10.3390/plants10020257.
In our search for novel plant-derived inhibitors of nitric oxide (NO) with potential for treating inflammatory diseases, the phytochemicals of fruits were investigated, leading to the isolation of one bicyclic nonane (), three menthene skeleton monoterpenoids (-), and two acyclic monoterpenoids ( and ). Their structures were identified using one- and two-dimensional nuclear magnetic resonance spectroscopy, and mass spectrometry. To the best of our knowledge, compounds - were obtained from the genus for the first time. All isolates were tested for their ability to inhibit lipopolysaccharide-stimulated NO overproduction in RAW264.7 cells. Compound was found to inhibit NO production. Western blotting analysis indicated that active compound can regulate inducible NO synthase expression. In addition, lipopolysaccharide-induced interleukin 1 beta and interleukin-6 overproduction was reduced in a concentration-dependent manner.
在我们寻找具有治疗炎症性疾病潜力的新型植物源一氧化氮（NO）抑制剂的过程中，对水果的植物化学成分进行了研究，从而分离出一种双环壬烷（）、三种薄荷烯骨架单萜类化合物（-）和两种无环单萜类化合物（和）。使用一维和二维核磁共振光谱以及质谱对它们的结构进行了鉴定。据我们所知，化合物 - 首次从属中获得。对所有分离物抑制RAW264.7细胞中脂多糖刺激的NO过量产生的能力进行了测试。发现化合物可抑制NO产生。蛋白质印迹分析表明活性化合物可调节诱导型NO合酶的表达。此外，脂多糖诱导的白细胞介素1β和白细胞介素6的过量产生以浓度依赖性方式减少。
