Physics and Chemistry of Nanostructures, Ghent University , Krijgslaan 281-S3, 9000 Ghent, Belgium.
ACS Appl Mater Interfaces. 2014 Jul 23;6(14):11714-23. doi: 10.1021/am502367b. Epub 2014 Jul 3.
We report on the synthesis of luminescent CdSe/CdS@SiO2 nanoparticles and their application to cell labeling. The main novelty of these nanoparticles is the use of newly developed "flash" CdSe/CdS quantum dots (QDs), which are obtained through a new fast and efficient synthesis method recently reported. These core-shell QDs are encapsulated in silica nanoparticles through a water-in-oil microemulsion process, resulting in CdSe/CdS@SiO2 nanoparticles with good morphology and controlled architecture. The main asset of these luminescent nanoparticles is their high photoluminescent quantum yield, which is equal to that of the original CdSe/CdS QDs and remains unchanged even after several months of storage in water. Thanks to the remarkable stability of their optical property in aqueous environment and to their low levels of toxicity, the high potential of these nanoparticles for long-term cell labeling is demonstrated.
我们报告了发光 CdSe/CdS@SiO2 纳米粒子的合成及其在细胞标记中的应用。这些纳米粒子的主要新颖之处在于使用了新开发的“闪蒸”CdSe/CdS 量子点（QDs），这些 QDs 是通过最近报道的一种新的快速高效合成方法获得的。这些核壳 QDs 通过油包水微乳液工艺封装在硅纳米粒子中，得到具有良好形态和可控结构的 CdSe/CdS@SiO2 纳米粒子。这些发光纳米粒子的主要优点是其具有高的光致荧光量子产率，与原始的 CdSe/CdS QDs 相等，即使在水中储存几个月后也保持不变。由于其在水相环境中光学性质的显著稳定性及其低毒性，这些纳米粒子在长期细胞标记方面具有很高的潜力。
