Moyano Daniel F, Saha Krishnendu, Prakash Gyan, Yan Bo, Kong Hao, Yazdani Mahdieh, Rotello Vincent M
Department of Chemistry, University of Massachusetts Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
ACS Nano. 2014 Jul 22;8(7):6748-55. doi: 10.1021/nn5006478. Epub 2014 Jul 11.
A protein corona is formed at the surface of nanoparticles in the presence of biological fluids, masking the surface properties of the particle and complicating the relationship between chemical functionality and biological effects. We present here a series of zwitterionic NPs of variable hydrophobicity that do not adsorb proteins at moderate levels of serum protein and do not form hard coronas at physiological serum concentrations. These particles provide platforms to evaluate nanobiological behavior such as cell uptake and hemolysis dictated directly by chemical motifs at the nanoparticle surface.
在生物流体存在的情况下，纳米颗粒表面会形成一层蛋白质冠层，掩盖颗粒的表面性质，并使化学功能与生物学效应之间的关系变得复杂。我们在此展示了一系列具有可变疏水性的两性离子纳米颗粒，它们在中等血清蛋白水平下不会吸附蛋白质，在生理血清浓度下也不会形成硬冠层。这些颗粒提供了平台，可用于评估直接由纳米颗粒表面化学基序决定的细胞摄取和溶血等纳米生物学行为。
